{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:48:51Z","timestamp":1760204931212},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,7,1]]},"abstract":"<jats:p> The properties of the conductive Cl- transport pathway underlying regulatory volume decrease (RVD) in human neutrophils were investigated using the whole cell patch-clamp technique. Cell swelling was induced during whole cell recordings by making the patch pipette solution hyperosmotic (approximately 20%) relative to the bath by addition of sucrose. Immediately after establishment of the whole cell configuration, no measurable Cl- currents were evident. Over a period of several minutes the outwardly rectifying Cl- current that developed displayed no apparent voltage dependence of activation and did not inactivate with time during voltage steps over the range of -80 to +80 mV. Reduction of Cl- currents by application of suction to the interior of the pipette implied that the swelling-induced Cl- channels are activated by membrane stretch. Based on reversal potential measurements, the volume-induced Cl- conductance was found to discriminate poorly among Cl-, Br-, I-, and NO3-, to possess a finite permeability to glucuronate (Pglucuronate\/PCl approximately 0.1) and to be impermeable to cations. Single-channel conductance was estimated to be 1.5 pS from analysis of the variance of membrane current fluctuations. The activated Cl- currents were blocked by 100 microM of the compound MK-447 analogue A (inhibitor constant Ki = 37 microM) and by 200 microM 3,5-diiodosalicylate, 500 microM 4-acetamido-4'-iodothiocyanostilbene-2,2'-disulfonic acid, and 200 microM UK-5099. These results suggest that the initial event triggering RVD in neutrophils may be activation of stretch sensitive Cl- channels in the plasma membrane. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.1.c156","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:52:11Z","timestamp":1514127131000},"page":"C156-C165","source":"Crossref","is-referenced-by-count":53,"title":["Whole cell Cl- currents in human neutrophils induced by cell swelling"],"prefix":"10.1152","volume":"265","author":[{"given":"J. S.","family":"Stoddard","sequence":"first","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, St. Louis,Missouri."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Steinbach","sequence":"additional","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, St. Louis,Missouri."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Simchowitz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, St. Louis,Missouri."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.1.C156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:21Z","timestamp":1718653341000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.1.C156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,7,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.1.C156"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.1.c156","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7,1]]}}}