{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T17:37:02Z","timestamp":1770831422414,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,7,1]]},"abstract":"<jats:p> To determine the molecular steps involved in the vasopressin-induced renal Na+ reabsorption, the patch-clamp technique was utilized to study the role of this hormone in the regulation of apical Na+ channels in renal epithelial A6 cells. Addition of arginine vasopressin (AVP) induced and\/or enhanced Na+ channel activity within 5 min of addition under cell-attached conditions. The AVP-induced channel activity was a reflection of both an increase in the average apparent channel number (0.2-1.7) and the percent open time (2-56%). Addition of the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine, the adenosine 3',5'-cyclic monophosphate (cAMP) analogues, 8-(4-chlorophenylthio)-cAMP and 8-bromo-cAMP, or forskolin elicited a comparable effect to that of AVP. The induced channels had similar properties to Na+ channels previously reported, including a channel conductance of 9 pS, Na(+)-to-K+ selectivity of 3-5:1, and high amiloride sensitivity. The cAMP-dependent protein kinase A (PKA) in the presence of ATP induced and\/or enhanced Na+ channel activity in excised inside-out patches with a change in average apparent channel number and percent open probability similar to those observed with either AVP or cAMP analogues in intact cells. Addition of activated pertussis toxin (100 ng\/ml) completely blocked the AVP- or PKA-induced Na+ channel activity in excised inside-out patches, whereas incubation of intact cells with the toxin completely prevented the effect of both activators. The data indicate that AVP mediates its effect through a cAMP-dependent pathway involving PKA activation whose target is the G protein pathway that regulates apical epithelial Na+ channel activity. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.1.c218","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:52:11Z","timestamp":1514127131000},"page":"C218-C223","source":"Crossref","is-referenced-by-count":39,"title":["Vasopressin and protein kinase A activate G protein-sensitive epithelial Na+ channels"],"prefix":"10.1152","volume":"265","author":[{"given":"A. G.","family":"Prat","sequence":"first","affiliation":[{"name":"Renal Unit, Massachusetts General Hospital, Charlestown 02129."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. A.","family":"Ausiello","sequence":"additional","affiliation":[{"name":"Renal Unit, Massachusetts General Hospital, Charlestown 02129."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. F.","family":"Cantiello","sequence":"additional","affiliation":[{"name":"Renal Unit, Massachusetts General Hospital, Charlestown 02129."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.1.C218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:44Z","timestamp":1718653364000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.1.C218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,7,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.1.C218"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.1.c218","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7,1]]}}}