{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:31:57Z","timestamp":1759941117980},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,7,1]]},"abstract":"<jats:p> Single-channel currents of the luminal membrane of marginal cells dissected from the guinea pig cochlea were investigated using the patch-clamp technique. Nonselective cation channels having a linear conductance of 27 pS were activated by depolarization, cytoplasmic Ca2+, and cytoplasmic acidification. Cytoplasmic ATP inactivated the channel. A mixture of 3-isobutyl-1-methylxanthine and forskolin activated a small-conductance Cl channel in the cell-attached mode. On excision in the inside-out mode, the Cl channel was inactivated, but it was reactivated by a cytoplasmic catalytic subunit of protein kinase A with ATP. This Cl channel had a linear conductance of 12 pS, and its activity was little affected by voltage. The sequence of permeation by anions was Br- &gt; Cl &gt; I-. The Cl channel blocker diphenylamine-2-carboxylic acid (3 mM) completely blocked the channel, but 5-nitro-2-(3-phenylpropylamino)-benzoic acid (50 microM) blocked it only partially. The above-mentioned characteristics are similar to those of the well-demonstrated Cl- channel, cystic fibrosis transmembrane regulator. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.1.c72","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:52:11Z","timestamp":1514127131000},"page":"C72-C78","source":"Crossref","is-referenced-by-count":35,"title":["Nonselective cation and Cl channels in luminal membrane of the marginal cell"],"prefix":"10.1152","volume":"265","author":[{"given":"H.","family":"Sunose","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Tohoku University School of Medicine,Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Tohoku University School of Medicine,Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Tohoku University School of Medicine,Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Tohoku University School of Medicine,Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Takasaka","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Tohoku University School of Medicine,Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.1.C72","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:45Z","timestamp":1718653365000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.1.C72"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,7,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.1.C72"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.1.c72","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7,1]]}}}