{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:25:33Z","timestamp":1790771133908,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,9,1]]},"abstract":"<jats:p> K efflux pathways responsible for regulatory volume decrease (RVD) were examined in bovine aortic endothelial cells. Hypotonic swelling produced a rapid and reversible threefold increase in bumetanide-insensitive 86Rb efflux. Swelling-activated 86Rb efflux was inhibited 43% when Cl was replaced with NO3, and this Cl-dependent efflux was inhibited by 1 mM furosemide. Neither Cl replacement nor furosemide inhibited the efflux stimulated by a Ca ionophore (A23187) in isotonic medium. Swelling-activated 86Rb efflux was also inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonate but not by dinitrostilbenedisulfonate. Cell swelling induced a volume-regulatory K loss that was incomplete in hypotonic medium but complete and more rapid when bumetanide was added or when cells were swollen isosmotically. K loss in the presence of bumetanide was partially blocked by furosemide. We conclude that two separate swelling-activated K fluxes mediate RVD in aortic endothelial cells: a Cl-dependent, furosemide-sensitive, but bumetanide-insensitive flux that is consistent with K-Cl cotransport, and a Cl-independent efflux that presumably is mediated by K channels. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.3.c763","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:42:13Z","timestamp":1514126533000},"page":"C763-C769","source":"Crossref","is-referenced-by-count":42,"title":["Swelling-activated K fluxes in vascular endothelial cells: volume regulation via K-Cl cotransport and K channels"],"prefix":"10.1152","volume":"265","author":[{"given":"P. B.","family":"Perry","sequence":"first","affiliation":[{"name":"Department of Medicine, Emory University School of Medicine, Atlanta,Georgia 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. C.","family":"O'Neill","sequence":"additional","affiliation":[{"name":"Department of Medicine, Emory University School of Medicine, Atlanta,Georgia 30322."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.3.C763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:42Z","timestamp":1718653362000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.3.C763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,9,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.3.C763"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.3.c763","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,9,1]]}}}