{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:07:12Z","timestamp":1718669232480},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,11,1]]},"abstract":"<jats:p> The beta-adrenergic agonist isoproterenol induced an increase in intracellular calcium concentration ([Ca2+]i) in rat submandibular granular ducts that was blocked by beta-adrenergic but not by alpha-adrenergic or muscarinic antagonists. This effect was only partially inhibited by the selective beta 1- and beta 2-adrenergic antagonists atenolol and ICI-118,551, but was completely blocked by the combination of the two, suggesting the involvement of multiple (or atypical) beta-adrenergic receptor subtypes. The response to isoproterenol was mimicked by forskolin, 3-isobutyl-1-methylxanthine, and dibutyryl adenosine 3',5'-cyclic monophosphate, but it was blocked by protein kinase inhibitors. The response of [Ca2+]i to isoproterenol was sustained in Ca(2+)-replete replete medium but transient in Ca(2+)-free medium, indicating the involvement of both Ca2+ entry and release from intracellular stores. However, isoproterenol stimulation produced no increase in ductal inositol phosphate levels. In addition, isoproterenol was still able to increase [Ca2+]i after the carbachol-induced depletion of inositol 1,4,5-trisphosphate (IP3)-sensitive calcium stores. We conclude that isoproterenol, acting through cAMP, releases Ca2+ from an IP3-insensitive intracellular store in salivary granular ducts. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.5.c1356","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:47:42Z","timestamp":1514126862000},"page":"C1356-C1362","source":"Crossref","is-referenced-by-count":29,"title":["Beta-adrenergic stimulation and cAMP mobilize Ca2+ from an IP3-insensitive pool in rat submandibular granular ducts"],"prefix":"10.1152","volume":"265","author":[{"given":"J. P.","family":"Dehaye","sequence":"first","affiliation":[{"name":"Department of General Biochemistry, Free University of Brussels,Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. H.","family":"Valdez","sequence":"additional","affiliation":[{"name":"Department of General Biochemistry, Free University of Brussels,Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of General Biochemistry, Free University of Brussels,Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.5.C1356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:39Z","timestamp":1718653359000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.5.C1356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,11,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.5.C1356"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.5.c1356","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,11,1]]}}}