{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:03:54Z","timestamp":1760295834209},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,12,1]]},"abstract":"<jats:p> In dose-dependent fashion, extracellular ATP reduces the increase in cytosolic Ca2+ concentration ([Ca2+]o) due to mobilization of cellular Ca2+ stores by both epinephrine [half-maximal inhibitory concentration (IC50) = 35.7 +\/- 12.9 microM; Hill coefficient (NH) = -2.0 +\/- 0.7, n = 8] and by carbachol (IC50 = 27.0 +\/- 7.0 microM, NH = -2.3 +\/- 0.7, n = 9). Inhibition is due to ATP4- but does not result from any emptying or inaccessibility of Ca2+ stores, which are readily mobilized by thapsigargin in the presence of ATP4-. Reduction of Ca2+ mobilization is rapid but is not due to direct interference by ATP with the interaction of carbachol or epinephrine with their respective cell surface receptors. A benzoyl derivative of ATP, 3'-O-(4-benzoyl) adenosine 5'-triphosphate (BZATP) is more potent than ATP in reducing [Ca2+]i due to mobilization of stored Ca2+ by either carbachol or epinephrine (IC50 for carbachol = 3.9 +\/- 0.4 microM, NH = -3.2 +\/- 0.5; IC50 for epinephrine = 3.8 +\/- 0.2, NH = -2.6 +\/- 0.7, n = 3) but GTP, UTP, ADP, and adenosine do not inhibit mobilization of stored Ca2+ by either carbachol or epinephrine. Neither ATP nor BZATP prevents the influx of extracellular Ca2+ stimulated by carbachol or epinephrine. These results suggest that ATP inhibits Ca2+ mobilization by autonomic neurotransmitters after occupation of P2Z purinoceptors. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.265.6.c1472","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:28:51Z","timestamp":1514125731000},"page":"C1472-C1478","source":"Crossref","is-referenced-by-count":25,"title":["Extracellular ATP prevents the release of stored Ca2+ by autonomic agonists in rat submandibular gland acini"],"prefix":"10.1152","volume":"265","author":[{"given":"T. W.","family":"Hurley","sequence":"first","affiliation":[{"name":"Department of Child Health, School of Medicine, University ofMissouri-Columbia 65212."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. D.","family":"Shoemaker","sequence":"additional","affiliation":[{"name":"Department of Child Health, School of Medicine, University ofMissouri-Columbia 65212."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. P.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Department of Child Health, School of Medicine, University ofMissouri-Columbia 65212."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.265.6.C1472","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:41:00Z","timestamp":1718653260000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.265.6.C1472"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1993,12,1]]}},"alternative-id":["10.1152\/ajpcell.1993.265.6.C1472"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.265.6.c1472","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12,1]]}}}