{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:06:56Z","timestamp":1718669216348},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,1,1]]},"abstract":"<jats:p> Inhibition of Na(+)-K(+)-ATPase activates Na(+)-K(+)-2Cl- cotransporter activity in cultured ciliary epithelium. Am. J. Physiol. 266 (Cell Physiol. 35): C198-C205, 1994.--86Rb uptake experiments were conducted to measure Na(+)-K(+)-ATPase activity and Na(+)-K(+)-2Cl- cotransporter activity in a cell line derived from rabbit nonpigmented ciliary epithelium. The presence of a Na(+)-K(+)-2Cl- cotransporter was supported by the observation of a bumetanide-sensitive 86Rb uptake component that was dependent on the extracellular concentration of both sodium and chloride. Potassium influx mediated by the Na(+)-K(+)-2Cl- cotransporter and Na(+)-K(+)-ATPase accounted for approximately 46 and 33% of total potassium uptake, respectively, whereas both ouabain- and bumetanide-resistant uptake accounted for 9%. Inhibition of the Na(+)-K(+)-ATPase had a stimulatory effect on Na(+)-K(+)-2Cl- cotransporter activity, which was dependent on the extent and duration of Na(+)-K(+)-ATPase inhibition. Ouabain treatment stimulated the potassium (86Rb) efflux rate and reduced intracellular potassium ([K]i). Potassium channel blockers suppressed the ouabain-activated potassium efflux and inhibited the ouabain-induced activation of the Na(+)-K(+)-2Cl- cotransporter. We conclude that Na(+)-K(+)-ATPase inhibition leads to the opening of potassium channels, which exacerbates the depletion of cellular potassium; Na(+)-K(+)-2Cl- cotransporter stimulation caused by the fall of [K]i overrides the tendency of increased cellular sodium to inhibit the cotransporter. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.1.c198","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:34:48Z","timestamp":1514126088000},"page":"C198-C205","source":"Crossref","is-referenced-by-count":14,"title":["Inhibition of Na(+)-K(+)-ATPase activates Na(+)-K(+)-2Cl- cotransporter activity in cultured ciliary epithelium"],"prefix":"10.1152","volume":"266","author":[{"given":"J.","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, University of Louisville School ofMedicine, Kentucky 40292."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. A.","family":"Delamere","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Louisville School ofMedicine, Kentucky 40292."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Coca-Prados","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Louisville School ofMedicine, Kentucky 40292."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.1.C198","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:45Z","timestamp":1718653245000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.1.C198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.1.C198"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.1.c198","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1,1]]}}}