{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:28:21Z","timestamp":1771644501803,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,2,1]]},"abstract":"<jats:p> Distal colon from guinea pig was stimulated in vitro by aldosterone in Ussing chambers that allowed measurement of short-circuit current (Isc) and tissue conductance (Gt). The response to aldosterone was delayed by approximately 20 min and resulted in a negative Isc, consistent with K secretion. Approximately 1 h later the Isc began to increase and eventually became positive, consistent with subsequent stimulation of Na absorption. The Na-absorptive response could be inhibited by mucosal amiloride without altering the rate of K secretion. Similarly, K secretion could be inhibited by serosal bumetanide without altering Na absorption. In the presence of spironolactone, actinomycin D, or cycloheximide, aldosterone failed to stimulate both K secretion and Na absorption. A dose response to aldosterone provided an apparent Kd of 2.6 +\/- 0.5 nM, consistent with a high-affinity receptor coupled to this secretory response. Stimulation by the K secretagogue epinephrine did not produce an additive increase in K secretion, suggesting that the same cell type responds to both aldosterone and epinephrine and that the protein induced by aldosterone was not one of the membrane proteins responsible for K secretion. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.2.c552","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:13:44Z","timestamp":1514139224000},"page":"C552-C558","source":"Crossref","is-referenced-by-count":25,"title":["Aldosterone stimulates K secretion prior to onset of Na absorption in guinea pig distal colon"],"prefix":"10.1152","volume":"266","author":[{"given":"D. R.","family":"Halm","sequence":"first","affiliation":[{"name":"Department of Physiology, Ohio State University, Columbus 43210."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. T.","family":"Halm","sequence":"additional","affiliation":[{"name":"Department of Physiology, Ohio State University, Columbus 43210."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.2.C552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:21Z","timestamp":1718653221000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.2.C552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.2.C552"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.2.c552","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,1]]}}}