{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T09:57:24Z","timestamp":1758707844891},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,3,1]]},"abstract":"<jats:p> To characterize volume regulatory mechanisms, unidirectional Rb+ efflux and influx, unidirectional Cl- influx, and cell volume were measured in cultured human retinal pigment epithelium (HRPE). The HRPE was found to be capable of both regulatory volume increase (RVI), in response to a hypertonic challenge, and regulatory volume decrease (RVD), in response to a hypotonic challenge. Bumetanide-sensitive Rb+ influx increased almost threefold on incubation in a hypertonic (390 mosmol\/kgH2O) medium. Bumetanide-insensitive Rb+ influx was activated by hypotonic (190 mosmol\/kgH2O) challenge as well as by treatment with N-ethylmaleimide (NEM). Exposure to hypotonic media also activated unidirectional Cl- influx and unidirectional Rb+ efflux. Both the RVD and hypotonically activated Rb+ efflux were inhibited by the K(+)-channel blocker barium. On the other hand, hypotonically activated Rb+ influx was increased by barium treatment. In sum, the HRPE exhibits volume-sensitive transport mechanisms over a range of volumes from 190 to 390 mosmol\/kgH2O. Cultured HRPE possess hypertonically activated Na-K-Cl cotransport, hypotonically activated K-Cl cotransport, and a barium-inhibitable hypotonically activated K+ efflux pathway. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.3.c676","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:28:16Z","timestamp":1514140096000},"page":"C676-C683","source":"Crossref","is-referenced-by-count":29,"title":["Volume regulation in cultured cells derived from human retinal pigment epithelium"],"prefix":"10.1152","volume":"266","author":[{"given":"B. G.","family":"Kennedy","sequence":"first","affiliation":[{"name":"Department of Physiology, Northwest Center for Medical Education,Indiana University School of Medicine, Gary 46408."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.3.C676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:47Z","timestamp":1718653247000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.3.C676"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,3,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.3.C676"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.3.c676","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3,1]]}}}