{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T18:42:22Z","timestamp":1776019342914,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,4,1]]},"abstract":"<jats:p> Radioactive tracers and a modified capacitance-probe technique were used to characterize the mechanisms that mediate Cl and fluid absorption across the bullfrog retinal pigment epithelium (RPE)-choroid. In control (HCO3\/CO2) Ringer solution, 36Cl was actively absorbed (retina to choroid) at a mean rate of 0.34 mu eq.cm-2.h-1 (n = 34) and accounted for approximately 25% of the short-circuit current. Apical bumetanide (100 microM) or basal 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS; 1 mM) inhibited active Cl transport by 70 and 62%, respectively. Active Cl absorption was doubled, either by removing HCO3 from the bathing media or by elevating CO2 from 5 to 13%, and the increased flux was inhibited by apical bumetanide or basal DIDS. Open-circuit measurements of fluid absorption rate (Jv) and the net fluxes of 36Cl, 22Na, and 86Rb (K substitute) indicated that CO2-induced acidification stimulated NaCl and fluid absorption across the RPE. During acidification, bumetanide produced a twofold larger inhibition of Jv compared with control. Stimulation of net Cl absorption was most likely caused by inhibition of the the basolateral membrane intracellular pH-dependent Cl-HCO3 exchanger. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.4.c946","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:16:37Z","timestamp":1514139397000},"page":"C946-C956","source":"Crossref","is-referenced-by-count":33,"title":["Acidification stimulates chloride and fluid absorption across frog retinal pigment epithelium"],"prefix":"10.1152","volume":"266","author":[{"given":"J. L.","family":"Edelman","sequence":"first","affiliation":[{"name":"School of Optometry, University of California, Berkeley 94720."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Optometry, University of California, Berkeley 94720."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Miller","sequence":"additional","affiliation":[{"name":"School of Optometry, University of California, Berkeley 94720."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.4.C946","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:39:31Z","timestamp":1718653171000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.4.C946"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1994,4,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.4.C946"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.4.c946","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,4,1]]}}}