{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:43:12Z","timestamp":1771461792088,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,5,1]]},"abstract":"<jats:p> Aldosterone secretion from the adrenal glomerulosa (AG) cells is inhibited by atrial natriuretic peptide (ANP). Inasmuch as alterations in K+ conductance can modulate aldosterone secretion, the effect of ANP on intracellular K+ homeostasis was investigated. Intracellular K+ concentration ([K+]i) of AG cells was assessed by spectrofluorometry using the K(+)-sensitive dye, K(+)-binding benzofuran isophthalate. The resting value of [K+]i in AG cells was determined to be 120 +\/- 1.2 mM (n = 37) in a HCO3-free, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-buffered medium. Exposure of AG cells to ANP led to a dose-dependent, transient decrease in [K+]i, from 21 +\/- 3.2% (n = 7) at 100 pM to 31 +\/- 2.3% at 1 microM (n = 7). In the continued presence of ANP, a rapid recovery to near basal values of [K+]i was attained within 90 s. Measurements of membrane voltage using the potential sensitive dye 1-3(-sulfonatopropyl)-4-[beta-(-(di-n-butylamino)-6-naphthyl)vinyl ]- pyridinium betaine documented an accompanying change in membrane potential. Pretreatment of AG cells with barium (0.5 mM), tetraethylammonium (0.1 mM), charybdotoxin (100 nM), or ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid (0.5 mM) blunted the ANP-induced decrease in [K+]i. ANP-(7-23), the ANP-C-receptor selective agonist, which does not elevate guanosine 3',5'-cyclic monophosphate (cGMP) did not alter [K+]i in contrast to cGMP (50 microM), which did. We conclude that ANP via the activation of the ANP A receptor alters K+ homeostasis through a Ca(2+)-activatable K(+)-conductive pathway likely to be the maxi-K channel. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.5.c1357","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:11:04Z","timestamp":1514139064000},"page":"C1357-C1365","source":"Crossref","is-referenced-by-count":34,"title":["Atrial natriuretic peptide enhances activity of potassium conductance in adrenal glomerulosa cells"],"prefix":"10.1152","volume":"266","author":[{"given":"M. B.","family":"Ganz","sequence":"first","affiliation":[{"name":"Department of Medicine, Case Western Reserve University, ClevelandVeterans Affairs Medical Center, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J.","family":"Nee","sequence":"additional","affiliation":[{"name":"Department of Medicine, Case Western Reserve University, ClevelandVeterans Affairs Medical Center, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"Isales","sequence":"additional","affiliation":[{"name":"Department of Medicine, Case Western Reserve University, ClevelandVeterans Affairs Medical Center, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. Q.","family":"Barrett","sequence":"additional","affiliation":[{"name":"Department of Medicine, Case Western Reserve University, ClevelandVeterans Affairs Medical Center, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.5.C1357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:39:54Z","timestamp":1718653194000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.5.C1357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,5,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.5.C1357"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.5.c1357","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5,1]]}}}