{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T10:31:56Z","timestamp":1789813916533,"version":"4.0.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,5,1]]},"abstract":"<jats:p> We investigated the effect of homozygous genetic disruption of the murine cystic fibrosis transmembrane regulator (CFTR) gene on regulation of the rates of Na+ absorption and Cl- secretion by nasal epithelia in cystic fibrosis (CF) mice. The basal in vivo nasal potential difference (PD; -28.8 +\/- 1.8 mV, n = 10) and amiloride-sensitive PD (delta 13.8 +\/- 1.0 mV, n = 10) were raised in CF mice compared with controls [-7.8 +\/- 0.8 mV, n = 14 (basal); delta 4.5 +\/- 0.7 mV, n = 14 (amiloride)], consistent with raised Na+ transport. In vitro studies of freshly excised nasal epithelia confirmed that CF epithelia exhibited a greater basal equivalent short-circuit current (Ieq; 63.5 +\/- 12 microA\/cm2, n = 15) vs. control (30.2 +\/- 7.2 microA\/cm2, n = 16) and amiloride-sensitive Ieq (delta 46.2 +\/- 12.5 microA\/cm2) vs. control (delta 11.3 +\/- 4.5 microA\/cm2). Tissue from normal mice failed to secrete Cl- in response to ionomycin (delta Ieq: -1.2 +\/- 1.9 microA\/cm2, n = 18), whereas CF murine tissue responded with a large rise in Ieq (delta 55.1 +\/- 19.1 microA\/cm2, n = 13). We conclude that CF murine nasal epithelia exhibit Na+ hyperabsorption, providing strong evidence for a regulatory link between CFTR and Na+ channel activity in airway epithelia. We speculate that upregulation of the Ca(2+)-mediated Cl- secretory pathway buffers the severity of airway disease in the CF mouse. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.5.c1478","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:11:04Z","timestamp":1514139064000},"page":"C1478-C1483","source":"Crossref","is-referenced-by-count":177,"title":["Hyperabsorption of Na+ and raised Ca(2+)-mediated Cl- secretion in nasal epithelia of CF mice"],"prefix":"10.1152","volume":"266","author":[{"given":"B. R.","family":"Grubb","sequence":"first","affiliation":[{"name":"Division of Pulmonary Diseases, University of North Carolina at ChapelHill 27599."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. N.","family":"Vick","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Diseases, University of North Carolina at ChapelHill 27599."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. C.","family":"Boucher","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Diseases, University of North Carolina at ChapelHill 27599."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.5.C1478","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:39:56Z","timestamp":1718653196000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.5.C1478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,5,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.5.C1478"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.5.c1478","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5,1]]}}}