{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:45:36Z","timestamp":1772898336300,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,6,1]]},"abstract":"<jats:p> We measured fluxes of radiotracers in Xenopus oocytes expressing the cystic fibrosis transmembrane conductance regulator (CFTR). Addition of adenosine 3',5'-cyclic monophosphate (cAMP)-elevating agents [forskolin and 3-isobutyl-1-methylxanthine (I\/F)] led to large increases in uptake of 36Cl, 125I, and 82Br into oocytes expressing wild-type CFTR or delta F508 CFTR but not sham-injected oocytes. I\/F also stimulated halide efflux from CFTR and delta F508 oocytes in the sequence Cl &gt; Br &gt; I. cAMP-induced increases in 36Cl efflux from delta F508 oocytes were approximately 20% of those in CFTR oocytes. Increases in halide efflux were blocked by diphenylamine-2-carboxylic acid but not by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The phorbol ester, phorbol 12-myristate 13-acetate, also stimulated 36Cl efflux from CFTR oocytes. ATP uptakes into CFTR and sham oocytes were similar, and both were reduced by I\/F. However, ATP uptake into I\/F-treated CFTR oocytes was slightly greater (approximately 40%) than into I\/F-treated sham oocytes. Urea uptake into CFTR and sham oocytes was similar and in both cases was increased by I\/F. However, the I\/F-induced increase in urea uptake into CFTR oocytes was significantly greater than for sham oocytes. I\/F stimulated formate uptake into CFTR oocytes but not into sham oocytes. Fluxes of 22Na, 86Rb, 35SO4, 32PO4, and mannitol were unaltered by expression and activation of CFTR. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.6.c1586","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:57:39Z","timestamp":1514138259000},"page":"C1586-C1593","source":"Crossref","is-referenced-by-count":8,"title":["Radiotracer studies of cystic fibrosis transmembrane conductance regulator expressed in Xenopus oocytes"],"prefix":"10.1152","volume":"266","author":[{"given":"T.","family":"Ohrui","sequence":"first","affiliation":[{"name":"Department of Physiology, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Skach","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Matsumoto-Pon","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Calayag","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Widdicombe","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of California, San Francisco94143."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.6.C1586","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:41:31Z","timestamp":1718653291000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.6.C1586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1994,6,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.6.C1586"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.6.c1586","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6,1]]}}}