{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:46:47Z","timestamp":1787701607357,"version":"build-2784847793"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,6,1]]},"abstract":"<jats:p> Angiotensin II has been reported to stimulate the proximal tubule Na-H antiporter by inhibition of adenylyl cyclase, and possibly by an adenosine 3',5'-cyclic monophosphate (cAMP)-independent mechanism. We examined the effect of angiotensin II on Na-H antiporter activity (JNa-H) in opossum kidney (OKP) cells, a proximal tubule-like cell line, whose Na-H antiporter resembles that of the proximal tubule apical membrane. We found that angiotensin II regulates JNa-H in a concentration-dependent manner similar to the proximal tubule, with angiotensin II concentrations &lt; 10(-8) M stimulating and &gt; 10(-8) M inhibiting JNa-H. The stimulatory effect of angiotensin II was blocked by 10(-8) M losartan and was pertussis toxin sensitive, suggesting mediation through an angiotensin II (AT1) receptor coupled to a pertussis toxin-sensitive G protein. Acute treatment with 10(-4) M 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) inhibited JNa-H by 30% and blocked angiotensin II-induced stimulation. However, angiotensin II (10(-12)-10(-6) M) did not inhibit basal, dopamine-stimulated, or forskolin-stimulated cAMP production measured in the presence of 3-isobutyl-1-methylxanthine (IBMX). In addition, angiotensin II had no effect on cAMP levels measured in the absence of IBMX. We conclude that angiotensin II at physiological concentrations stimulates JNa-H in OKP cells via a cAMP-independent mechanism mediated by an AT1 receptor and a pertussis toxin-sensitive G protein. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.6.c1603","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:57:39Z","timestamp":1514138259000},"page":"C1603-C1608","source":"Crossref","is-referenced-by-count":51,"title":["Angiotensin II stimulation of Na-H antiporter activity is cAMP independent in OKP cells"],"prefix":"10.1152","volume":"266","author":[{"given":"A.","family":"Cano","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Texas SouthwesternMedical Center, Dallas 75235-8856."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. T.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas SouthwesternMedical Center, Dallas 75235-8856."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Alpern","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas SouthwesternMedical Center, Dallas 75235-8856."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. A.","family":"Preisig","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas SouthwesternMedical Center, Dallas 75235-8856."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.6.C1603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:41:18Z","timestamp":1718653278000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.6.C1603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1994,6,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.6.C1603"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.6.c1603","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6,1]]}}}