{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:06:59Z","timestamp":1718669219516},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,6,1]]},"abstract":"<jats:p> Conventional whole cell voltage-clamp recording results in washout of the cardiac Ca2+ current (ICa) response to the beta-adrenergic agonist isoproterenol (Iso), for reasons which are not clear. When dose-response curves for the phosphodiesterase (PDE) inhibitor 3-isobutyl-1-methylxanthine (IBMX) were compared using perforated-patch vs. conventional whole cell recording in guinea pig ventricular myocytes, the conventional whole cell IBMX responses were unexpectedly larger than the perforated-patch responses. Furthermore, during conventional whole cell recording the response to repeated application of Iso declined rapidly, whereas the IBMX response initially increased and then declined. When pipette [Ca2+] was increased to 10(-7) M, conventional whole cell responses to 300 microM IBMX and 10(-9) M Iso were identical to perforated-patch responses. Thus loss of the Iso response during conventional whole cell recording seems to not be solely due to a washout of some constituent of the adenosine 3',5'-cyclic monophosphate pathway. We suggest that unphysiological intracellular [Ca2+] enhances the relative PDE activity and that this contributes to the rapid decline of the Iso response and the initial enhancement of the IBMX response. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.266.6.c1619","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:57:39Z","timestamp":1514138259000},"page":"C1619-C1627","source":"Crossref","is-referenced-by-count":14,"title":["Perforated-patch recording does not enhance effect of 3-isobutyl-1-methylxanthine on cardiac calcium current"],"prefix":"10.1152","volume":"266","author":[{"given":"A.","family":"Kawamura","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Illinois atChicago 60612-7342."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. M.","family":"Wahler","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Illinois atChicago 60612-7342."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.266.6.C1619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:44Z","timestamp":1718653244000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.266.6.C1619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1994,6,1]]}},"alternative-id":["10.1152\/ajpcell.1994.266.6.C1619"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.266.6.c1619","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6,1]]}}}