{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:48:51Z","timestamp":1760204931808},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,8,1]]},"abstract":"<jats:p> Recently, we showed that NO2- increases gap junction (GJ) permeability and synchronizes intracellular Ca2+ concentration oscillations in pancreatic acini (Loessburg et al., J. Biol. Chem. 268: 19769-19775, 1993). NO2- is also an end product of nitric oxide (NO) production and metabolism. Because of the effect of NO2- on GJ permeability and the possible importance of NO2- in NO metabolism and cytotoxicity, we used pancreatic acinar cells and intracellular pH (pHi) measurements to study the interaction of nitrogen oxides and NO2- with cellular proteins. Exposing cells to NO2- resulted in a concentration-dependent cytosolic acidification. The acidification did not require the transport of NO2- and was not mediated by diffusion of HNO2. Because the acidification was prevented by CO2-HCO3- and inhibition of carbonic anhydrase, it is possible that other nitrogen oxides present in a solution containing NO2- enter the cells by diffusion and interact with OH- or H2O to stably acidify the cytosol. NO2- itself is shown to be transported by the HCO3- transporters present in the plasma membrane. Thus manipulation of the cellular Cl- gradient and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) were used to show Cl-\/NO2- exchange, whereas stimulation of external Na(+)-dependent amiloride-insensitive and DIDS-sensitive pHi increase in acidified cells was used to demonstrate a Na(+)-(NO2-)n cotransport. Hence NO2- can be a convenient substitute for HCO3- when studying HCO3- transport in an open system. The studies also show that cellular levels of nitrogen oxides and NO2- can be modulated by the cellular HCO3(-)-buffering system.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1994.267.2.c385","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:59:45Z","timestamp":1514138385000},"page":"C385-C393","source":"Crossref","is-referenced-by-count":10,"title":["Transport and interaction of nitrogen oxides and NO2 with CO2-HCO3- transporters in pancreatic acini"],"prefix":"10.1152","volume":"267","author":[{"given":"H.","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Physiology and Internal Medicine, University of TexasSouthwestern Medical Center, Dallas 75235."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Internal Medicine, University of TexasSouthwestern Medical Center, Dallas 75235."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Ujiie","sequence":"additional","affiliation":[{"name":"Department of Physiology and Internal Medicine, University of TexasSouthwestern Medical Center, Dallas 75235."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A.","family":"Star","sequence":"additional","affiliation":[{"name":"Department of Physiology and Internal Medicine, University of TexasSouthwestern Medical Center, Dallas 75235."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Muallem","sequence":"additional","affiliation":[{"name":"Department of Physiology and Internal Medicine, University of TexasSouthwestern Medical Center, Dallas 75235."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.267.2.C385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:29Z","timestamp":1718653229000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.267.2.C385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,8,1]]}},"alternative-id":["10.1152\/ajpcell.1994.267.2.C385"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.267.2.c385","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8,1]]}}}