{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:03:38Z","timestamp":1791291818675,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,8,1]]},"abstract":"<jats:p> Chinese hamster ovary (CHO-K1) cells were stably transfected with prolactin (PRL) receptor cDNA. These cells (CHO-E32) expressed the long form of functional PRL receptor. Using microfluorimetric and patch-clamp techniques, we have investigated the effects of PRL on intracellular Ca2+ concentration ([Ca2+]i) and membrane ion conductances. Exposure of CHO-E32 cells to 5 nM PRL resulted in an increase in [Ca2+]i. Two types of response were observed: 1) a stimulation of Ca2+ entry and 2) an intracellular Ca2+ mobilization. As PRL inhibited voltage-activated Ca2+ current, the PRL-induced Ca2+ increase does not involve voltage-activated Ca2+ channels. PRL also increased a charybdotoxin-sensitive Ca(2+)-dependent K+ conductance. Simultaneous measurements showed that PRL hyperpolarized the membrane potential before increasing intracellular Ca2+ levels. In voltage clamp, hyperpolarizing voltage steps were associated with increased Ca2+ concentrations, whereas depolarizing voltage steps decreased [Ca2+]i. Cell-free patch-clamp experiments showed that PRL directly stimulates K+ channel activity. Our results suggest the existence of a regulatory complex involving a protein kinase tightly associated with the Ca(2+)-activated K+ channels and that PRL stimulates these channels by means of the activation of protein kinase. The resulting hyperpolarization stimulates Ca2+ entry, probably through voltage-insensitive nonspecific channels. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.267.2.c554","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:59:45Z","timestamp":1514138385000},"page":"C554-C562","source":"Crossref","is-referenced-by-count":22,"title":["Early effects of PRL on ion conductances in CHO cells expressing PRL receptor"],"prefix":"10.1152","volume":"267","author":[{"given":"N.","family":"Prevarskaya","sequence":"first","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Skryma","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Vacher","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Daniel","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Bignon","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Djiane","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Dufy","sequence":"additional","affiliation":[{"name":"Laboratory of Neurophysiology, University of Bordeaux II,France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.267.2.C554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:11Z","timestamp":1718653211000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.267.2.C554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,8,1]]}},"alternative-id":["10.1152\/ajpcell.1994.267.2.C554"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.267.2.c554","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8,1]]}}}