{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:04:16Z","timestamp":1778274256297,"version":"3.51.4"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,10,1]]},"abstract":"<jats:p> Insulin augments the activity of Na(+)-K(+)-adenosinetriphosphatase (ATPase) in skeletal muscles. This study shows that when furosemide- and bumetanide-inhibitable 86Rb+ uptake is measured in the skeletal muscle-like BC3H1 cell line, insulin and insulin-like growth factor I (IGF-I) activate a loop diuretic-sensitive K+ and Cl- transport system but have no effect on Na(+)-K(+)-ATPase. The insulin-stimulated K+ transport system is extracellular Na+ concentration ([Na+]o) independent and extracellular Cl- concentration ([Cl-]o) dependent. Na(+)-independent K(+)-Cl- cotransport systems have been identified in other cells, but their sensitivity to insulin or growth factors has not been described. The affinities of the insulin-stimulated K+ uptake in BC3H1 cells for K+ (0.9 +\/- 0.1 mM) and loop diuretics (5.9 x 10(-7) and 10(-7) M for furosemide and bumetanide, respectively) are higher than those of K(+)-Cl- cotransporters in other cells. Thus the insulin-stimulated K+ and Cl- transport system in BC3H1 seems kinetically different from K(+)-Cl- cotransporters in other cells. Insulin and IGF-I may activate a unique K(+)-Cl- cotransporter or activate a [Na+]o-independent K(+)-Cl- cotransport mode of Na(+)-K(+)-Cl- cotransporter in BC3H1 cells. <\/jats:p>","DOI":"10.1152\/ajpcell.1994.267.4.c932","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:45:38Z","timestamp":1514137538000},"page":"C932-C939","source":"Crossref","is-referenced-by-count":24,"title":["Insulin activates furosemide-sensitive K+ and Cl- uptake system in BC3H1 cells"],"prefix":"10.1152","volume":"267","author":[{"given":"E.","family":"Weil-Maslansky","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Hebrew University of Jerusalem, School ofPharmacy, Faculty of Medicine, Israel."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Gutman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebrew University of Jerusalem, School ofPharmacy, Faculty of Medicine, Israel."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sasson","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebrew University of Jerusalem, School ofPharmacy, Faculty of Medicine, Israel."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.267.4.C932","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:03Z","timestamp":1718653203000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.267.4.C932"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1994,10,1]]}},"alternative-id":["10.1152\/ajpcell.1994.267.4.C932"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.267.4.c932","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10,1]]}}}