{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:50:48Z","timestamp":1790319048170,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1994,12,1]]},"abstract":"<jats:p> To characterize Cl(-)-dependent NH4+ transport mechanisms in renal medullary thick ascending limb (MTAL), intracellular pH (pHi) and membrane potential (PD) were monitored with use of 2',7'-bis(carboxyethyl)- 5(6)-carboxyfluorescein and 3,3'-dipropylthiadicarbocyanine, respectively, in suspensions of rat MTAL tubules in CO2-free media. Exposure of MTAL cells to 4 mM NH4Br caused, after an initial cell alkalinization due to NH3 entry, an NH4(+)-induced fall in pHi that was approximately 67% less pronounced in Cl(-)-free than in Cl(-)-containing media. The following experiments were performed in the presence of 1 microM amiloride to block the MTAL NH4+ conductance. When cells were preincubated in a Cl(-)-free gluconate medium in which K+ and Cl- conductances are greatly reduced, abrupt addition of 100 mN N-methyl-D-glucamine (NMDG)-Cl had no effect on cell PD and pHi in the absence of ammonia, but acutely acidified the cells by approximately 0.2 pH units in the presence of 4 mM NH4Br, which thus indicated nonelectrogenic (NMDG-Cl)-dependent NH4+ influx. The latter also occurred in a Cl(-)-free thiocyanate medium in which the Cl- conductance was blocked by 0.1 mM diphenylamine-2-carboxylate (DPC). An NMDG-Cl- dependent NH4(+)-induced fall in pHi was reduced approximately 33% by 10 mM Ba+, approximately 84% by 0.1 mM bumetanide, and 100% by 1.5 mM furosemide, whereas 1 mM hydrochlorothiazide had no effect; inhibition by Ba+ was observed even in the presence of 0.1 mM verapamil added to block both K+ channels and K+\/NH4+ antiport.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1994.267.6.c1607","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:48:50Z","timestamp":1514141330000},"page":"C1607-C1615","source":"Crossref","is-referenced-by-count":47,"title":["Cl(-)-dependent NH4+ transport mechanisms in medullary thick ascending limb cells"],"prefix":"10.1152","volume":"267","author":[{"given":"H.","family":"Amlal","sequence":"first","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 356,Universite Pierre et Marie Curie, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Paillard","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 356,Universite Pierre et Marie Curie, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bichara","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 356,Universite Pierre et Marie Curie, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1994.267.6.C1607","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:39:14Z","timestamp":1718653154000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1994.267.6.C1607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1994,12,1]]}},"alternative-id":["10.1152\/ajpcell.1994.267.6.C1607"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1994.267.6.c1607","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,12,1]]}}}