{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:21:12Z","timestamp":1757704872099},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:p> The relationship between substrate and metabolism in vascular smooth muscle has been investigated by studying the acute energetic effects caused by the creatine analogue beta-guanidinopropionic acid (beta-GPA) on porcine carotid arteries using 31P-nuclear magnetic resonance (NMR). Porcine carotid arteries were superfused for 12 h with Krebs-Henseleit buffer at 22 degrees C, containing 50 mM beta-GPA, and either 11 mM glucose or 5 mM pyruvate as substrate. beta-GPA enters the cells and becomes phosphorylated by creatine kinase to produce beta-GPA-P. Perfusion with beta-GPA leads to the formation of NMR observable beta-GPA-P (after 2.5 h). The appearance of beta-GPA-P with time was significantly greater when glucose was used as substrate. To differentiate between oxidative and glycolytic metabolism in the phosphorylation of beta-GPA, 1 mM cyanide was included in the perfusion buffer containing 50 mM beta-GPA and 11 mM glucose. No phosphocreatine (PCr) was observed with these conditions, and there was a small but significant decrease in ATP concentration ([ATP]) compared with glucose perfusion without cyanide (0.56 +\/- 0.02 to 0.47 +\/- 0.02 mumol\/g wet wt), that was greater than the concentration with pyruvate as substrate (0.25 +\/- 0.03 mumol\/g wet wt). Thus the [ATP] during cyanide treatment is maintained with glycolytic metabolism. Despite the relatively high [ATP], accumulation of beta-GPA-P only occurred over a much slower time course ( &gt; 10 h) than without cyanide.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1995.268.3.c628","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:49:55Z","timestamp":1514141395000},"page":"C628-C635","source":"Crossref","is-referenced-by-count":24,"title":["Metabolite utilization and compartmentation in porcine carotid artery: a study using beta-guanidinopropionic acid"],"prefix":"10.1152","volume":"268","author":[{"given":"E. A.","family":"Boehm","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. K.","family":"Radda","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Oxford, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1995.268.3.C628","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:38:18Z","timestamp":1718653098000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1995.268.3.C628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3,1]]}},"alternative-id":["10.1152\/ajpcell.1995.268.3.C628"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1995.268.3.c628","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,1]]}}}