{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:47:02Z","timestamp":1773658022227,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1995,6,1]]},"abstract":"<jats:p> Triad vesicles were isolated from normal (N) and homozygous malignant hyperthermia-susceptible (MHS) porcine skeletal muscle, and two types of sarcoplasmic reticulum Ca2+ release were investigated: 1) polylysine-induced Ca2+ release (direct stimulation of the junctional foot protein), and 2) depolarization-induced Ca2+ release (stimulation of the junctional foot protein via the dihydropyridine receptor). At submaximal concentrations of polylysine, the rates of induced Ca2+ release from the MHS triads were greater than from normal triads. The T tubules of polarized triads were depolarized by the K(+)-to-Na+ ionic replacement protocol. Higher grades of T-tubule depolarization resulted in higher rates of Ca2+ release from both MHS and normal triads but, when compared at a given grade of T-tubule depolarization, the release rate was always greater from the MHS than from normal triads. Thus the activity of the SR Ca2+ release channel is always higher in MHS than in normal muscle at a given submaximal dose of release trigger. This difference is observed when the channel is stimulated directly by polylysine or indirectly via a depolarization-induced activation of the T-tubule dihydropyridine receptor. <\/jats:p>","DOI":"10.1152\/ajpcell.1995.268.6.c1381","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:37:37Z","timestamp":1514140657000},"page":"C1381-C1386","source":"Crossref","is-referenced-by-count":22,"title":["Altered E-C coupling in triads isolated from malignant hyperthermia-susceptible porcine muscle"],"prefix":"10.1152","volume":"268","author":[{"given":"R.","family":"el-Hayek","sequence":"first","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Yano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Antoniu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Mickelson","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. F.","family":"Louis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ikemoto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul 55108, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1995.268.6.C1381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:38:42Z","timestamp":1718653122000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1995.268.6.C1381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,6,1]]}},"alternative-id":["10.1152\/ajpcell.1995.268.6.C1381"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1995.268.6.c1381","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,6,1]]}}}