{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:54:41Z","timestamp":1770486881679,"version":"3.49.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1995,8,1]]},"abstract":"<jats:p> Net cation movements were measured in low-density sickle red blood cells (SS RBC) in the presence and absence of oxygen. External Ca2+ (Ca2+o) partially inhibited deoxygenation-induced fluxes of both Na+ and K+. Deoxygenation-induced Na+ influx was reduced by 2 mM Ca2+o to 0.71 +\/- 0.04 (SE) of its value in Ca(2+)-free solutions, whereas this ratio was 0.90 +\/- 0.05 for K+ efflux (P &lt; 0.01 by paired t-test). Because Ca2+o inhibited Na+ influx more than K+ efflux, net cation loss in deoxygenated SS RBC was higher in the presence of Ca2+o. In separate experiments, Ca2+o reduced deoxygenation-induced Na+ influx to 0.66 +\/- 0.03 of its Ca(2+)-free value compared with 0.77 +\/- 0.03 for Rb+ influx (P &lt; 0.001), indicating relative selectivity of this effect for Na+ over Rb+. However, this effect is not specific for Ca2+ because other divalent cations also inhibited deoxygenation-induced Na+ and K+ fluxes. Under the conditions of these experiments, no evidence for K+ channel activation was found, indicating that K+ loss measured in deoxygenated SS RBC was mediated by the deoxygenation-induced pathway. These studies show that in the presence of Ca2+o deoxygenation-induced Na+ influx and K+ efflux are unbalanced. This pathway can, therefore, mediate cation loss and contribute directly to cellular dehydration in SS RBC. <\/jats:p>","DOI":"10.1152\/ajpcell.1995.269.2.c403","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:29:02Z","timestamp":1514140142000},"page":"C403-C409","source":"Crossref","is-referenced-by-count":38,"title":["Deoxygenation-induced cation fluxes in sickle cells. IV. Modulation by external calcium"],"prefix":"10.1152","volume":"269","author":[{"given":"C. H.","family":"Joiner","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Cincinnati College ofMedicine, Children's Hospital Medical Center, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Cincinnati College ofMedicine, Children's Hospital Medical Center, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S.","family":"Franco","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Cincinnati College ofMedicine, Children's Hospital Medical Center, Ohio, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1995.269.2.C403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:38:55Z","timestamp":1718653135000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1995.269.2.C403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,8,1]]}},"alternative-id":["10.1152\/ajpcell.1995.269.2.C403"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1995.269.2.c403","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8,1]]}}}