{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:37:18Z","timestamp":1773657438054,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1995,10,1]]},"abstract":"<jats:p> When swollen in hypotonic media, HL-60 cells exhibit a regulatory volume decrease (RVD) response as a result of net losses of K+ and Cl-. This is primarily caused by a dramatic increase in Cl- permeability, which may reflect the opening of volume-sensitive channels (11). To test this hypothesis, we measured volume-activated Cl- currents in HL-60 cells using the patch-clamp technique. The whole cell Cl- conductance (in nS\/pF at 100 mV) increased from 0.09 +\/- 0.06 to 1.15 +\/- 0.19 to 1.64 +\/- 0.40 as the tonicity (in mosmol\/kgH2O) of the external medium was decreased from 334 to 263 to 164, respectively. Cl- currents showed no significant inactivation during 800-ms pulses. Current-voltage curves exhibited outward rectification and were identical at holding potentials of 0 or -50 mV, suggesting that the gating of the channels is voltage independent. The selectivity sequence, based on permeability ratios (PX\/PCl) calculated from the shifts of the reversal potentials, was SCN- &gt; I- approximately NO3- &gt; Br- &gt; Cl- &gt;&gt; gluconate. 4-Acetamido-4'- isothiocyanostilbene-2,2'-disulfonic acid (SITS; 0.5 mM) inhibits HL-60 Cl- channels in a voltage-dependent manner, with approximately 10-fold increased affinity at potentials greater than +40 mV. Voltage-dependent blockade by SITS indicates that the binding site is located near the outside, where it senses 20% of the membrane potential. These Cl- channels were also inhibited in a voltage-independent manner by the oxonol dye bis-(1,3-dibutylbarbituric acid)pentamethine oxonol [diBA-(5)-C4] with a concentration that gives half inhibition (IC50) of 1.8 microM at room temperature. A similar apparent IC50 value (1.2 microM) was observed for net 36Cl- efflux into a Cl(-)-free hypotonic medium at 21 degrees C. It seems likely, therefore, that the volume-activated Cl- channels are responsible for the net Cl- efflux during RVD. These Cl- channels have properties similar to the \u201cmini-Cl-\u201d channels described in lymphocytes and neutrophils and are strongly inhibited by low concentrations of diBA-(5)-C4. <\/jats:p>","DOI":"10.1152\/ajpcell.1995.269.4.c1063","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:24:08Z","timestamp":1514139848000},"page":"C1063-C1072","source":"Crossref","is-referenced-by-count":9,"title":["Volume-activated chloride channels in HL-60 cells: potent inhibition by an oxonol dye"],"prefix":"10.1152","volume":"269","author":[{"given":"J.","family":"Arreola","sequence":"first","affiliation":[{"name":"Department of Dental Research, University of Rochester, New York 14642, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. R.","family":"Hallows","sequence":"additional","affiliation":[{"name":"Department of Dental Research, University of Rochester, New York 14642, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. A.","family":"Knauf","sequence":"additional","affiliation":[{"name":"Department of Dental Research, University of Rochester, New York 14642, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1995.269.4.C1063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:39:09Z","timestamp":1718653149000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1995.269.4.C1063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,10,1]]}},"alternative-id":["10.1152\/ajpcell.1995.269.4.C1063"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1995.269.4.c1063","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,10,1]]}}}