{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T19:31:13Z","timestamp":1774121473266,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1995,10,1]]},"abstract":"<jats:p> The effect of adenosine 3',5'-cyclic monophosphate (cAMP) on intracellular Na+ concentration ([Na+]i) was studied in primary cultured vascular smooth muscle cells from rat aorta. [Na+]i was measured using digital imaging of cells loaded with the Na(+-)sensitive fluorescent dye sodium-bonding benzofuran isophthalate. The cAMP level was raised by 1) the membrane-permeable cAMP derivative 8-bromoadenosine 3',5'-cyclic monophosphate, 2) the combination of the adenylate cyclase activator forskolin and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, and 3) the beta-adrenoceptor agonist isoproterenol. All three methods caused a dose-dependent continuous rise in [Na+]i during 40-60 min of observations. A rise in [Na+]i may be caused by stimulation of the Na+ influx and\/or inhibition of Na+ efflux; therefore, the involvement of both mechanisms was studied. Elevation of the cAMP level had no effect on Na+ influx, measured as the rate of rise of [Na+]i when Na+ efflux was inhibited with 1 mM ouabain. In contrast, elevation of the cAMP level attenuated Na+ efflux, measured as the rate of decline of [Na+]i in Na(+)-loaded cells exposed to Na(+)-free medium. cAMP-induced inhibition of Na+ efflux was not observed when the Na+ pump was inhibited; therefore, cAMP inhibits the Na+ pump-mediated component of Na+ efflux. Agents that raise the cAMP level also inhibited, in a dose-dependent fashion, ouabain-sensitive 86Rb uptake in rat aortic rings. The latter observation confirms that the cAMP-induced inhibition of the Na+ pump occurs both in cultured cells and in the native tissue.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1995.269.4.c884","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:24:08Z","timestamp":1514139848000},"page":"C884-C891","source":"Crossref","is-referenced-by-count":32,"title":["cAMP evokes a rise in intracellular Na+ mediated by Na+ pump inhibition in rat aortic smooth muscle cells"],"prefix":"10.1152","volume":"269","author":[{"given":"M. L.","family":"Borin","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1995.269.4.C884","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:39:03Z","timestamp":1718653143000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1995.269.4.C884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,10,1]]}},"alternative-id":["10.1152\/ajpcell.1995.269.4.C884"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1995.269.4.c884","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,10,1]]}}}