{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T13:52:56Z","timestamp":1747749176556},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,2,1]]},"abstract":"<jats:p> Current responses to caffeine in single hepatocytes dissociated from adult rat liver were investigated with the conventional whole cell patch-recording configuration. Caffeine produced a sustained inward current (Icaf) with increasing conductance at a holding potential of -40 mV. The reversal potential of Icaf was close to the Cl- equilibrium potential. Icaf was not affected by the internal perfusion of 1,2-bis(2-amino-phenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) or Cs+, whereas the Ca(2+)-activated K+ outward current elicited by A-23187 was inhibited by intracellular BAPTA or Cs+. A 1 mM 3-isobutyl-1-methylxanthine (IBMX) was about equipotent to 1 mM caffeine in inducing the current. Icaf was not modulated by the external application of N-(2-[methylamino]ethyl)-5-isoquinolinesulfonamide (H-8), a cyclic nucleotide-dependent protein kinase inhibitor, or intracellular perfusion with guanosine-5'-O-(2-thiodiphosphate) (GDP beta S) or guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S). It was concluded that caffeine induced an increase in membrane Cl- conductance without utilizing the rise of intracellular free Ca2+ or adenosine 3',5'-cyclic monophosphate (cAMP) and without mediating G protein, suggesting the possible existence of caffeine receptor-Cl- channel complexes on liver plasma membrane. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.270.2.c508","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:14:19Z","timestamp":1514142859000},"page":"C508-C513","source":"Crossref","is-referenced-by-count":5,"title":["Caffeine-induced chloride current in dissociated rat hepatocytes"],"prefix":"10.1152","volume":"270","author":[{"given":"Y.","family":"Yamashita","sequence":"first","affiliation":[{"name":"Department of Physiology, Kyushu University Faculty of Medicine,Fukuoka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Akaike","sequence":"additional","affiliation":[{"name":"Department of Physiology, Kyushu University Faculty of Medicine,Fukuoka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.270.2.C508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:36:39Z","timestamp":1718652999000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.270.2.C508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,2,1]]}},"alternative-id":["10.1152\/ajpcell.1996.270.2.C508"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.270.2.c508","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2,1]]}}}