{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:18:59Z","timestamp":1771643939824,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,3,1]]},"abstract":"<jats:p> To determine whether gluco- and mineralocorticoids have specific actions on Na+-K+-ATPase gene expression in vascular tissue, we used Northern blot analysis to compare the effects of dexamethasone (Dex) and aldosterone (Aldo) on Na+-K+-ATPase alpha1 and beta1-subunit mRNA expression in cultured vascular smooth muscle cells from rat aortae. Dex at 10(-6)M increased alpha1 -mRNA level 2.5-fold at 24 h and beta1-mRNA level 9.9-fold at 12 h. Aldo at 10(-6)M increased alpha1-mRNA 2.7-fold at 48 h and beta1-mRNA level 10.9-fold at 6 h. The half-maximal stimulation of both alpha1 and beta1-mRNA levels occurred at a concentration of 5-7 X 10(-9)M Dex, whereas it occurred at a concentration of 2-3 X 10(-9)M Aldo. The glucocorticoid receptor antagonist RU-38486 inhibited both Dex- and Aldo-mediated induction of beta1-mRNA. The mineralocorticoid receptor antagonist spironolactone inhibited Aldo-mediated induction of beta1-mRNA, whereas it had no effect on Dex-mediated induction of beta1-mRNA. Removal of Na+ from the extracellular medium (isosmotic replacement with choline) caused no effect on Dex-mediated induction beta1-mRNA, whereas it inhibited Aldo-mediated induction of beta1-mRNA. Addition of a specific inhibitor of the Na+\/H+ exchange, ethylisopropylamiloride, had no effect on Dex-mediated induction of beta1-mRNA, whereas it resulted in a significant inhibition of Aldo-mediated induction of beta1-mRNA. We conclude that 1) both Dex and Aldo induce Na+-K+-ATPase alpha1- and beta1-mRNA expression in a time- and dose-dependent manner; 2) Dex-mediated induction of beta1-mRNA occurs only through glucocorticoid receptors, whereas Aldo-mediated induction of beta1-mRNA occurs through both gluco- and mineralocorticoid receptors; and 3) Dex-mediated induction of beta1-mRNA occurs through Na+-independent mechanisms, whereas Aldo-mediated induction of beta1-mRNA, at least in part, occurs through Na+-dependent mechanisms, including stimulation of the Na+\/H+ exchange. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.270.3.c731","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:04:07Z","timestamp":1514142247000},"page":"C731-C739","source":"Crossref","is-referenced-by-count":22,"title":["Differential regulation of Na+-K+-ATPase gene expression by corticosteriods in vascular smooth muscle cells"],"prefix":"10.1152","volume":"270","author":[{"given":"S.","family":"Muto","sequence":"first","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Nemoto","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ohtaka","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Yamaki","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nagano","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Jichi Medical School, Minamikawachi,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.270.3.C731","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:35:08Z","timestamp":1718652908000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.270.3.C731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3,1]]}},"alternative-id":["10.1152\/ajpcell.1996.270.3.C731"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.270.3.c731","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,3,1]]}}}