{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:07:41Z","timestamp":1718669261208},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,3,1]]},"abstract":"<jats:p> Prior studies show that ileal HCO3- secretion is of crypt origin, possibly involving Na+-HCO3- cotransport. To test for the latter, we isolated crypt cells from guinea pig ileum and determined effects of medium HCO3-, Na+, K+, disulfonic stilbenes, and gramicidin on intracellular pH [pHi;2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein fluorescence], cell volume (electronic sizing), and Na+ efflux from 22Na+ -preloaded cells. Ileal crypt cells alkalinized when placed in sodium gluconate-HCO3- medium containing N-5-methyl-5-isobutyl amiloride (1 microM), bumetanide (10 microM) and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (250 microM which blocks Cl-\/HCO3- exchange but not Na+ dependent HCO3- uptake). Depolarization with either gramicidin (50 microM) or 50 mM K+ caused a further 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS)-inhibitable increase in pHi. Gramicidin also caused SITS-inhibitable cell swelling. Both gramicidin effects were Na+ dependent: at 0 mM Na+, gramicidin acidified and did not alter cell volume; at 25 mM, gramicidin also acidified; at 90 and 140 mM, gramicidin alkalinized and induced cell swelling. HCO3- -dependent SITS-inhibitable Na+ efflux from 22Na+ -preloaded cells was also seen. We conclude that ileal crypt cells engage in electrogenic Na+ -HCO3- symport. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.270.3.c786","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:04:07Z","timestamp":1514142247000},"page":"C786-C793","source":"Crossref","is-referenced-by-count":10,"title":["Sodium-bicarbonate cotransport in guinea pig ileal crypt cells"],"prefix":"10.1152","volume":"270","author":[{"given":"R. J.","family":"MacLeod","sequence":"first","affiliation":[{"name":"Department of Pediatrics, McGill Univeristy-Montreal Children'sHospital Research Institute, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Redican","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, McGill Univeristy-Montreal Children'sHospital Research Institute, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Lembessis","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, McGill Univeristy-Montreal Children'sHospital Research Institute, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, McGill Univeristy-Montreal Children'sHospital Research Institute, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Field","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, McGill Univeristy-Montreal Children'sHospital Research Institute, Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.270.3.C786","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:35:31Z","timestamp":1718652931000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.270.3.C786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3,1]]}},"alternative-id":["10.1152\/ajpcell.1996.270.3.C786"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.270.3.c786","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,3,1]]}}}