{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T05:01:21Z","timestamp":1775365281559,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,4,1]]},"abstract":"<jats:p> Egression of atrial natriuretic peptide (ANP)-stimulated guanosine 3', 5'-cyclic monophosphate (cGMP) was compared with that of isoproterenol-stimulated adenosine 3', 5'-cyclic monophosphate (cAMP) in a rabbit collecting duct cell line transformed with a temperature-sensitive strain of simian virus 40 (SV40). At 39.5 degrees C (inactivated large T), cells exhibit major features of principal cells, whereas at 33 degrees C (functional large T) they lose most of their specific properties. When cells were grown on plastic at 39.5 degrees C, both cyclic nucleotides were predominantly released extracellularly via probenecid-sensitive carriers. Probenecid (3mM) reduced the ratios of extracellular cGMP and cAMP by 84 and 70%, respectively. The amount of extracellular cGMP or cAMP ws linearly correlated with the time integral of the intracellular cyclic nucleotide, suggesting first-order kinetics. The apparent first-order rate constant (k) was sixfold greater for cGMP (0.139 +\/- 0.037 min-1, n = 3 experiments) than for cAMP (0.022 +\/- 0.003(-1), n = 3 experiments). 3-Isobutyl-1-methylxanthine markedly inhibited extrusion of cGMP (k = 0.022 +\/- 0.003 min-1), whereas that of cAMP was unchanged. When cells were grown on filters at 39.5 degrees C, both nucleotides were predominantly released in the apical medium but with a greater polarity for cGMP (83 +\/- 4%, n = 6 experiments) than for cAMP (60 +\/- 6%, n = 3 experiments) and a prevailing apical localization of the probenecid-sensitive carrier. Activation of SV40 large T at 33 degrees C did not alter cyclic nucleotide transport characteristics but abolished the polarity of probenecid-sensitive cyclic nucleotide extrusion. These results suggest a physiological role for luminal cGMP in the rabbit collecting duct and a specific effect of large T on the probenecid-sensitive carrier polarity. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.270.4.c1051","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:24:26Z","timestamp":1514136266000},"page":"C1051-C1060","source":"Crossref","is-referenced-by-count":21,"title":["ANP-stimulated cGMP egression in renal principal cells: abrogation of polarity by SV40 large T"],"prefix":"10.1152","volume":"270","author":[{"given":"V.","family":"Millul","sequence":"first","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 64, Hopital Tenon, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Prie","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 64, Hopital Tenon, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Geniteau-Legendre","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 64, Hopital Tenon, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Verpont","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 64, Hopital Tenon, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Baudouin","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 64, Hopital Tenon, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M.","family":"Ronco","sequence":"additional","affiliation":[{"name":"Institut National de la Sante et de la Recherche Medicale Unite 64, Hopital Tenon, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.270.4.C1051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:36:40Z","timestamp":1718653000000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.270.4.C1051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,4,1]]}},"alternative-id":["10.1152\/ajpcell.1996.270.4.C1051"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.270.4.c1051","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,4,1]]}}}