{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T03:47:44Z","timestamp":1773805664766,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,6,1]]},"abstract":"<jats:p> The Na+\/H+ antiport and Na(+)-HCO3- coinflux carrier contribute to recovery from intracellular acidosis in cardiac tissue. The effects of angiotensin II (10(-12)-10(-6) M) on H+ fluxes after intracellular acid loading and during reperfusion after myocardial ischemia have been investigated in the isovolumic, Langendorff-perfused ferret heart. Intracellular pH (pHi) was estimated using 31P nuclear magnetic resonance (NMR) spectroscopy from the chemical shift of intracellular deoxyglucose-6-phosphate or inorganic phosphate. Angiotensin II produced concentration-dependent stimulation (maximum at 10(-6) M: 67%) of 5-(N-ethyl-N-isopropyl)amiloride (EIPA)-sensitive Na(+)-dependent of H+ efflux consistent with stimulation of the Na+\/H+ antiport. Half-maximal stimulation of H+ efflux occurred at approximately 10(-9) M, which is close to the dissociation constant of the cardiac angiotensin AT1 receptor. Stimulation via this receptor was confirmed with the nonpeptide AT1 receptor blocker, GR-117289. Angiotensin II had less pronounced effects on HCO3(-)-dependent pHi recovery after acid loading with no effect on pHi recovery after intracellular alkalosis. During reperfusion, angiotensin II significantly increased H+ extrusion but impaired contractile recovery. The results support the hypothesis that angiotensin II facilitates H+ extrusion in the heart. This may help maintain physiological homeostasis, but the hypothesized obligated Na+ influx could exacerbate cellular dysfunction during reperfusion. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.270.6.c1687","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:30:10Z","timestamp":1514140210000},"page":"C1687-C1694","source":"Crossref","is-referenced-by-count":24,"title":["Angiotensin II stimulates sodium-dependent proton extrusion in perfused ferret heart"],"prefix":"10.1152","volume":"270","author":[{"given":"A. A.","family":"Grace","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Metcalfe","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. L.","family":"Weissberg","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. W.","family":"Bethell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. I.","family":"Vandenberg","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, United Kingdom."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.270.6.C1687","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:35:57Z","timestamp":1718652957000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.270.6.C1687"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,6,1]]}},"alternative-id":["10.1152\/ajpcell.1996.270.6.C1687"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.270.6.c1687","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,6,1]]}}}