{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T15:24:32Z","timestamp":1782833072972,"version":"3.54.5"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,6,1]]},"abstract":"<jats:p> Genomic clones including the 5' flanking regions of the AQP2 (aquaporin 2) gene were isolated, and the promoter region was examined by transiently transfecting a promoter-luciferase reporter fusion gene into renal cultured epithelial cells. An orientation specific promoter for the AQP2 gene was found within the proximal 3 kb of 5'-flanking region. Minimal basal promoter activity of the AQP2 gene was found within 198 bp upstream from the transcription start site by deletion analysis. Sequencing the transcriptionally active region revealed a typical TATA box, adenosine 3',5'-cyclic monophosphate (cAMP) responsive element (CRE) and three putative CCAAT boxes in the proximal 1.2-kb region. Significantly, a GATA motif, AP1, AP2, and SP1 transcriptional factor consensus sites were also found in this region. Exposure to cAMP-enhancing agents (1 nM vasopressin or 20 mM forskolin and 250 mM 3-isobutyl-1-methylxanthine) showed that these agents increased luciferase activity in a parallel fashion, suggesting that vasopressin-induced AQP2 gene transcription is mediated through increases in intracellular cAMP in at least one renal cell type, the LLC-PK1 cells. The mechanism of cAMP responsiveness of AQP2 gene transcription was further studied using a series of deletion mutants in renal epithelial cells and other cell types. The cAMP regulatory motifs were shown to exist in a 50-bp sequence between -340 and -290 (containing CRE) and a 65-bp sequence (containing an AP2 site) between -150 and the ATG start site in LLC-PK1 cells. In rat inner medullary collecting duct (IMCD) cells, the cAMP regulatory motifs also exist in a 50-bp sequence between -340 and -290 (containing CRE) and in a 10-bp sequence between -160 and -150 (containing an SP1 site). These separate regions may cooperate to confer full cAMP inducibility to the AQP2 gene in a cell-specific manner <\/jats:p>","DOI":"10.1152\/ajpcell.1996.270.6.c1695","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T18:30:10Z","timestamp":1514140210000},"page":"C1695-C1702","source":"Crossref","is-referenced-by-count":138,"title":["cAMP motifs regulating transcription in the aquaporin 2 gene"],"prefix":"10.1152","volume":"270","author":[{"given":"S.","family":"Hozawa","sequence":"first","affiliation":[{"name":"Renal Unit, Massachusetts General Hospital, Boston 02114, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. J.","family":"Holtzman","sequence":"additional","affiliation":[{"name":"Renal Unit, Massachusetts General Hospital, Boston 02114, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"Ausiello","sequence":"additional","affiliation":[{"name":"Renal Unit, Massachusetts General Hospital, Boston 02114, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.270.6.C1695","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:36:26Z","timestamp":1718652986000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.270.6.C1695"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,6,1]]}},"alternative-id":["10.1152\/ajpcell.1996.270.6.C1695"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.270.6.c1695","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,6,1]]}}}