{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:11:14Z","timestamp":1790835074852,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,7,1]]},"abstract":"<jats:p> The immunosuppressants cyclosporin A (CyA), FK-506, and rapamycin (RAP) have multiple actions on target cells that appear to be mediated by interaction of drug-binding protein complexes. Both FK-506 and CyA, but not RAP, inhibit the Ca2(+)-dependent phosphatase, calcineurin, and in so doing have been found to inhibit Na(+)-K(+)-ATPase activity in various nephron segments. Of interest, FK-506 and RAP, but not CyA, are bound by the steroid receptor-associated FK-506-binding heat shock protein of 56 kDa, HSP56. To determine the physiological effect of this interaction on a steroid-mediated phenomenon, the effect of these agents on steroid-mediated Na+ transport in A6 cells was investigated. Aldosterone stimulation of Na+ transport and Na(+)-K(+)-ATPase activity are significantly inhibited by prolonged incubation with FK-506 and RAP. Although CyA inhibits basal Na(+)-K(+)-ATPase activity, it has no effect on aldosterone-induced Na+ transport or the aldosterone-induced increase in Na(+)-K(+)-ATPase activity. FK-506 inhibits the aldosterone-induced synthesis of G alpha i-3 protein but has no effect on glucocorticoid receptor number as quantified by Western blotting. The results suggest that FK-506 and RAP inhibit steroid-mediated Na+ transport at some pretranslational site. The common interaction of these agents with the steroid receptor-associated HSP56 might account for these findings. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.1.c194","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:05:26Z","timestamp":1514142326000},"page":"C194-C202","source":"Crossref","is-referenced-by-count":16,"title":["FK-506 and rapamycin but not cyclosporin inhibit aldosterone-stimulated sodium transport in A6 cells"],"prefix":"10.1152","volume":"271","author":[{"given":"M. D.","family":"Rokaw","sequence":"first","affiliation":[{"name":"Laboratory of Epithelial Cell Biology, University of Pittsburg Medical Center, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"West","sequence":"additional","affiliation":[{"name":"Laboratory of Epithelial Cell Biology, University of Pittsburg Medical Center, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. M.","family":"Palevsky","sequence":"additional","affiliation":[{"name":"Laboratory of Epithelial Cell Biology, University of Pittsburg Medical Center, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Laboratory of Epithelial Cell Biology, University of Pittsburg Medical Center, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.1.C194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:37:30Z","timestamp":1718653050000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.1.C194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,7,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.1.C194"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.1.c194","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7,1]]}}}