{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T20:35:31Z","timestamp":1789763731299,"version":"4.0.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,9,1]]},"abstract":"<jats:p> The regulation of the receptor-G protein-phospholipase C (PLC) cascade was investigated in rat myometrium at midgestation (day 12) and at term (day 21) comparatively to the estrogen-treated tissue (day 0). Carbachol-mediated generation of [3H]inositol phosphates was insensitive to pertussis toxin and was enhanced at days 12 and 21 two- and threefold, respectively, with no alteration of muscarinic sites (M3 subtype). A similar increase could be detected in the production of inositol 1,4,5-trisphosphate, indicating the stimulation of a PLC degrading phosphatidylinositol 4,5-bisphosphate. AlF4- also enhanced PLC activation during gestation, suggesting pregnancy-related regulations that bypass receptor activation. Immunoreactive G proteins, Gq alpha and G11 alpha, and PLC-beta 3 were detected in all myometrial preparations. The amount of PLC-beta 3 was similar in day 0 and day 21 myometrium, although decreasing by 75% at midgestation. Of significance was the increased amount of Gq alpha in day 12 and day 21 myometrium (3- and 2-fold, respectively) which coincided with the enhanced phosphoinositide breakdown. The upregulation of Gq alpha may contribute to the enhanced PLC activity during pregnancy and at term. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.3.c895","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:00:33Z","timestamp":1514145633000},"page":"C895-C904","source":"Crossref","is-referenced-by-count":42,"title":["Modulation of phospholipase C pathway and level of Gq alpha\/G11 alpha in rat myometrium during gestation"],"prefix":"10.1152","volume":"271","author":[{"given":"S.","family":"Lajat","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique Unite de Recherche Associee 1131, Universite Paris Sud, Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Tanfin","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique Unite de Recherche Associee 1131, Universite Paris Sud, Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Guillon","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique Unite de Recherche Associee 1131, Universite Paris Sud, Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Harbon","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique Unite de Recherche Associee 1131, Universite Paris Sud, Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.3.C895","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:34:54Z","timestamp":1718652894000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.3.C895"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,9,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.3.C895"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.3.c895","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9,1]]}}}