{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:43:18Z","timestamp":1774564998166,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,9,1]]},"abstract":"<jats:p> Neutrophil adhesion to the vascular endothelium is enhanced during tissue ischemia and\/or inflammation, conditions that are associated with tissue acidosis. This study examined the effects of hypercarbic acidosis (10 or 20% CO2) and of hypocarbic alkalosis (0% CO2) on human neutrophil CD18 and human aortic endothelial cell intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin expression quantified by flow cytometry. Acidosis with 20% CO2 for 4 h decreased ICAM-1 to 60.6 +\/- 9.7% of control. In contrast, alkalosis with 0% CO2 for 4 h enhanced ICAM-1 expression to 143.8 +\/- 10.1% of control. There was no pH dependence of VCAM-1 or E-selectin expression. Tumor necrosis factor-alpha (TNF-alpha; 10 ng\/ml) increased endothelial ICAM-1, E-selectin, and VCAM-1; under these conditions, acidosis with 20% CO2 blunted both ICAM-1 and E-selectin surface expression compared with 5% CO2-, TNF-alpha-treated cells. Hypercarbic acidosis with 20% CO2 increased neutrophil CD18 expression and enhanced neutrophil adhesion. This latter effect was inhibited by neutrophil pretreatment with an anti-CD18 monoclonal antibody. In contrast, when only endothelial cells were preincubated with the hypercarbic buffer, neutrophil adhesion diminished to 55.6 +\/- 7.8% of control. The results suggest that acidosis generated during tissue ischemia\/inflammation may induce CD18-mediated neutrophil adhesion despite a decrease in ICAM-1 expression. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.3.c962","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T20:00:33Z","timestamp":1514145633000},"page":"C962-C970","source":"Crossref","is-referenced-by-count":65,"title":["pH dependence of neutrophil-endothelial cell adhesion and adhesion molecule expression"],"prefix":"10.1152","volume":"271","author":[{"suffix":"Jr","given":"C. V.","family":"Serrano","sequence":"first","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Fraticelli","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Paniccia","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Teti","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Noble","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Corda","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Faraggiana","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. C.","family":"Ziegelstein","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Zweier","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Capogrossi","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Science, Gerontology Research Center, National Institute on Aging, National Institutes of Health, Baltimore, Maryland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.3.C962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:35:35Z","timestamp":1718652935000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.3.C962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,9,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.3.C962"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.3.c962","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9,1]]}}}