{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:44:50Z","timestamp":1761597890044},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,11,1]]},"abstract":"<jats:p> Ferrets have high-Na+ and low-K+ erythrocytes (113 and 5.4 mmol\/l cell water) due to the lack of Na(+)-K+ pumps. Because ferret erythrocytes have a high capacity for Na(+)-K(+)-2Cl- cotransport, the present study was undertaken to evaluate cell volume-related changes in cotransport activity and its role in volume regulation. With cell shrinkage, Na(+)-K(+)-2Cl- cotransport is activated about twofold. A large bumetanide-insensitive Na+ uptake component that has not yet been described is found in shrunken erythrocytes. Its inhibition by amiloride (concn inhibiting 50% of maximal response = 12 microM) and the Na+ dependence of amiloride-sensitive extracellular pH changes measured in cells suspended in hypertonic unbuffered medium indicate that this flux represents Na+\/H+ exchange. Shrinkage activation of both transporters follows a time lag of approximately 3 min and also requires normal levels of ATP. ATP depletion inhibits Na(+)-K(+)-2Cl- cotransport even at normal cell volume. Both transporters are partially inhibited by the protein kinase inhibitors staurosporine and K252a, and activators of protein kinases A and C do not affect transport. Okadaic acid inhibition of protein phosphatases activates Na(+)-K(+)-2Cl- cotransport to its maximal activity (same after shrinkage), but shrinkage and okadaic acid activation are not additive. In contrast, okadaic acid activates Na+\/H+ exchange even in shrunken cells. These results indicate that cell shrinkage activates Na(+)-K(+)-2Cl- cotransport and Na+\/H+ exchange probably by phosphorylation processes. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.5.c1603","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:59:53Z","timestamp":1514145593000},"page":"C1603-C1611","source":"Crossref","is-referenced-by-count":16,"title":["Na(+)-K(+)-2Cl- cotransport, Na+\/H+ exchange, and cell volume in ferret erythrocytes"],"prefix":"10.1152","volume":"271","author":[{"given":"H.","family":"Mairbaurl","sequence":"first","affiliation":[{"name":"Department of Sports Medicine, University of Heidelberg,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Herth","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, University of Heidelberg,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.5.C1603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:37:07Z","timestamp":1718653027000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.5.C1603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1996,11,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.5.C1603"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.5.c1603","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,11,1]]}}}