{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T14:42:08Z","timestamp":1759588928183},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,12,1]]},"abstract":"<jats:p> Studies from our laboratory have demonstrated rapid (&lt; 1 min) nongenomic activation of K+ recycling and Na+\/H+ exchange by mineralocorticoids in human colonic epithelium, and studies from other laboratories have demonstrated rapid effects of aldosterone on intracellular Ca2+ concentration ([Ca2+]i) in endothelial cells. Here a rapid nongenomic effect of aldosterone on [Ca2+]i is demonstrated in the human colonic epithelial cell line T84. Aldosterone induced a rapid increase in [Ca2+]i within approximately 2 min. The rise in [Ca2+]i after aldosterone appears to result from the activation of a Ca2+ influx pathway, inasmuch as 1) no increase in [Ca2+]i was observed with aldosterone when cells were bathed in Ca(2+)-free Krebs solution and 2) emptying of the intracellular Ca2+ stores by thapsigargin was not enhanced by addition of aldosterone to extracellular Ca(2+)-free solution. In contrast, the Ca2+ response to aldosterone, in the presence of 2 mM Ca2+ in the external bathing solution, was not decreased after intracellular Ca2+ stores were emptied by thapsigargin. Other mineralocorticoid hormones increased [Ca2+]i, whereas the glucocorticoid hydrocortisone failed to increase [Ca2+]i. These results demonstrate the existence of a mineralocorticoid-specific Ca(2+)-signaling pathway in human colonic T84 (crypt) epithelial cells. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.6.c1935","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:29:42Z","timestamp":1514143782000},"page":"C1935-C1941","source":"Crossref","is-referenced-by-count":37,"title":["Rapid effects of steroid hormones on free intracellular calcium in T84 colonic epithelial cells"],"prefix":"10.1152","volume":"271","author":[{"given":"C. M.","family":"Doolan","sequence":"first","affiliation":[{"name":"Wellcome Trust Cellular Physiology Research Unit, Department of Physiology, University College, Cork, Ireland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. J.","family":"Harvey","sequence":"additional","affiliation":[{"name":"Wellcome Trust Cellular Physiology Research Unit, Department of Physiology, University College, Cork, Ireland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.6.C1935","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:35:48Z","timestamp":1718652948000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.6.C1935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,12,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.6.C1935"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.6.c1935","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12,1]]}}}