{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:28:38Z","timestamp":1770910118684,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,1,1]]},"abstract":"<jats:p> The inhibitory effect of myoplasmic Mg2+ on Ca2+ release from the sarcoplasmic reticulum (SR) was examined in mechanically skinned skeletal muscle fibers from pigs of different ryanodine-receptor (RyR) genotypes. In fibers from pigs homozygous for the normal RyR allele, the free Mg2+ concentration ([Mg2+]) had to be lowered from the normal resting level of 1 to approximately 0.1 mM to induce Ca2+ release and a force response. Fibers from pigs heterozygous or homozygous for the RyR allele associated with malignant hyperthermia (MH) needed only a smaller reduction in free [Mg2+] to induce Ca2+ release (reduction to 0.1-0.2 and &gt; or = 0.2 mM, respectively). Dantrolene (20 microM) counteracted the effect of this reduced Mg2+ inhibition in MH muscle. The response of muscle fiber bundles to the caffeine-halothane contracture test in the three genotypes correlated well with the responsiveness of single fibers to reduced [Mg2+]. Thus the abnormal responsiveness of MH muscle to various stimuli may largely result from the reduced ability of myoplasmic Mg2+ to inhibit Ca2+ release from the SR. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.272.1.c203","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:32:22Z","timestamp":1514143942000},"page":"C203-C211","source":"Crossref","is-referenced-by-count":66,"title":["Reduced Mg2+ inhibition of Ca2+ release in muscle fibers of pigs susceptible to malignant hyperthermia"],"prefix":"10.1152","volume":"272","author":[{"given":"V. J.","family":"Owen","sequence":"first","affiliation":[{"name":"School of Zoology, La Trobe University, Bundoora, Victoria,Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. L.","family":"Taske","sequence":"additional","affiliation":[{"name":"School of Zoology, La Trobe University, Bundoora, Victoria,Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. D.","family":"Lamb","sequence":"additional","affiliation":[{"name":"School of Zoology, La Trobe University, Bundoora, Victoria,Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1997.272.1.C203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:32:25Z","timestamp":1718652745000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1997.272.1.C203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1,1]]}},"alternative-id":["10.1152\/ajpcell.1997.272.1.C203"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1997.272.1.c203","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1,1]]}}}