{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T10:16:33Z","timestamp":1751019393644},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:p> We examined the effect of physiological concentrations of urea (100-500 mM) on Na-K-2Cl cotransport in cultured cells from mouse medullary thick ascending limb (mTAL). Urea acutely inhibited bumetanide-sensitive K influx in mTAL cells in a concentration-dependent fashion, with a statistically significant inhibition (19%) at 100 mM and 86% inhibition at 500 mM. The effect of urea was entirely reversible and was blocked by prior treatment with okadaic acid, a phosphatase inhibitor, suggesting that urea exerts its action upstream of the phosphorylation-dephosphorylation step. Cell volume was unchanged in the presence of 500 mM urea. The number of [3H]bumetanide binding sites, a measure of the number of functioning cotransporter sites, was decreased in the presence of urea, and the decrease in bumetanide binding was proportional to the decrease in bumetanide-sensitive K influx. Urea also stimulated the Ba-sensitive swelling-activated K efflux from mTAL cells. Thus urea, in concentrations that prevail in the renal medulla, alters ion transport in mTAL cells. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.272.2.c615","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:36:54Z","timestamp":1514133414000},"page":"C615-C621","source":"Crossref","is-referenced-by-count":12,"title":["Urea inhibits Na-K-2Cl cotransport in medullary thick ascending limb cells"],"prefix":"10.1152","volume":"272","author":[{"given":"D. M.","family":"Kaji","sequence":"first","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, Bronx, NewYork 10468, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, Bronx, NewYork 10468, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Parker","sequence":"additional","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, Bronx, NewYork 10468, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1997.272.2.C615","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:33:06Z","timestamp":1718652786000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1997.272.2.C615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,2,1]]}},"alternative-id":["10.1152\/ajpcell.1997.272.2.C615"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1997.272.2.c615","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2,1]]}}}