{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:06:59Z","timestamp":1718669219368},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,5,1]]},"abstract":"<jats:p> Submandibular enzymatic vasoconstrictor (SEV, rK9) induces vascular contraction and platelet aggregation by a mechanism requiring intact enzymatic activity. On the basis of a published report demonstrating growth-promoting enzymatic activity in an extract of the rat submandibular gland, we hypothesized that SEV would affect DNA synthesis. Recombinant SEV (rSEV), expressed in a baculovirus system, increased DNA synthesis 3- to 25-fold in Chinese hamster lung (CCL39) fibroblasts and in rabbit and rat vascular smooth muscle cells in a dose-dependent manner dose eliciting 50% of maximal response: 0.1-1 nM); this effect was inhibited by pertussis toxin (PTX). Inactive rSEV failed to enhance DNA synthesis. In CCL39 fibroblasts, rSEV increased total phosphoinositol (PI) formation (6- to 10-fold at 10 nM), which was inhibited 49% by PTX; it was also partially inhibited by the tyrosine kinase inhibitor genistein (33%) but was not affected by the protein kinase C inhibitor bisindolylmaleimide. These results show that rSEV increases DNA synthesis and PI formation in mesenchymal cells in a dose- and enzymatic activity-dependent manner through a pathway partially mediated by a PTX-sensitive G protein. Thus SEV can induce growth-associated responses, perhaps through a protease-activated receptor mechanism. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.272.5.c1578","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:23:29Z","timestamp":1514132609000},"page":"C1578-C1585","source":"Crossref","is-referenced-by-count":0,"title":["Submandibular enzymatic vasoconstrictor increases DNA and phosphoinositol synthesis by mesenchymal cells"],"prefix":"10.1152","volume":"272","author":[{"given":"L. A.","family":"Carbini","sequence":"first","affiliation":[{"name":"Hypertension and Vascular Research Division, Henry Ford Hospital,Detroit, Michigan 48202, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. G.","family":"Scicli","sequence":"additional","affiliation":[{"name":"Hypertension and Vascular Research Division, Henry Ford Hospital,Detroit, Michigan 48202, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1997.272.5.C1578","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:32:05Z","timestamp":1718652725000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1997.272.5.C1578"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1997,5,1]]}},"alternative-id":["10.1152\/ajpcell.1997.272.5.C1578"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1997.272.5.c1578","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,5,1]]}}}