{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:18:02Z","timestamp":1792225082347,"version":"4.4.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,6,1]]},"abstract":"<jats:p> Purinergic receptors play an important role in regulating Cl- secretion in epithelial cells. To explore further the role of these receptors in the intestine, we utilized the human intestinal epithelial cell line, Caco-2, grown on permeable membrane supports and assayed for Cl- secretion by measuring the short-circuit current (Isc). Stimulation of Isc by extracellular nucleotides could be detected by day 4 and increased by day 10 postseeding. The magnitude of stimulation of Isc at 10 microM in cells at day 10 was UTP &gt; ATP &gt; UDP &gt; &gt; 2-methylthioadenosine 5'-triphosphate (2-MeS-ATP) = ADP on the apical side and UTP = 2-MeS-ATP = ATP &gt; ADP &gt; &gt; UDP on the basolateral side. Cross-desensitization studies suggested that two different receptors are expressed in the apical membrane, a P2U purinoceptor and a uridine nucleotide receptor. Two different receptors are also expressed in the basolateral membrane, a P2U receptor and another that reacts with both 2-MeS-ATP and ADP. This latter receptor has an unusual pharmacological profile, with a reactivity for 2-MeS-ATP &gt; ADP but not for ATP. Responses to purinergic receptor agonists were inhibited by pretreatment with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, thapsigargin, or quinine. Thus we suggest that an increase in intracellular Ca2+ and subsequent opening of Ca(2+)-activated K+ channel play a role in increasing driving force for Cl- to exit across the apical membrane. The role of the cystic fibrosis transmembrane conductance regulator as a Cl- exit pathway on the apical membrane was also established. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.272.6.c1862","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:21:17Z","timestamp":1514132477000},"page":"C1862-C1870","source":"Crossref","is-referenced-by-count":48,"title":["Role of purinergic receptors in chloride secretion in Caco-2 cells"],"prefix":"10.1152","volume":"272","author":[{"given":"C. N.","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. S.","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. M.","family":"Schwiebert","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Hanaoka","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. E.","family":"Guggino","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. B.","family":"Guggino","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1997.272.6.C1862","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:31:13Z","timestamp":1718652673000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1997.272.6.C1862"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,6,1]]}},"alternative-id":["10.1152\/ajpcell.1997.272.6.C1862"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1997.272.6.c1862","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,1]]}}}