{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T22:27:29Z","timestamp":1747780049623},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,7,1]]},"abstract":"<jats:p> We investigated pinacidil-activated K+ currents in vascular smooth muscle cells (VSMC) from human coronary arteries with the patch-clamp method. In 19 of 54 VSMC, pinacidil (1 and 20 microM) induced a large, nonrectifying, outward current [IK(ATP)] and increased voltage-dependent outward K+ currents [IK(Ca)] positive to voltages of -25 mV. The pinacidil-induced (1 microM) IK(ATP) was blocked by glibenclamide (3 microM) but was not affected by iberiotoxin (100-300 nM). Pinacidil activated up to 150 functionally active ATP-dependent K+ channels (KATP channels) per cell with a single-channel conductance of approximately 17 pS at physiological membrane potentials (between -80 and -30 mV) and K+ gradients (6 mM\/130 mM). In 26 of 54 VSMC, on the other hand, pinacidil (1-20 microM) failed to induce IK(ATP) but increased IK(Ca). This current was completely blocked by iberiotoxin (100-300 nM) and tetraethylammonium (1 mM) but not by glibenclamide (3 microM). The single-channel conductance of the channel underlying IK(Ca) was approximately 150 +\/- 16 pS between -10 and +30 mV, consistent with large-conductance, maxi Ca(2+)-activated, K+ channels (BKCa channels). We conclude that pinacidil is a nonselective K+ channel opener targeting KATP and BKCa channels. Furthermore, the conductance of KATP channels in human coronary arteries is likely to be small under physiological conditions. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.273.1.c161","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:12:33Z","timestamp":1514131953000},"page":"C161-C171","source":"Crossref","is-referenced-by-count":27,"title":["Effects of pinacidil on K+ channels in human coronary artery vascular smooth muscle cells"],"prefix":"10.1152","volume":"273","author":[{"given":"R.","family":"Bychkov","sequence":"first","affiliation":[{"name":"Franz Volhard Clinic, Virchow University Hospitals, Berlin,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gollasch","sequence":"additional","affiliation":[{"name":"Franz Volhard Clinic, Virchow University Hospitals, Berlin,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ried","sequence":"additional","affiliation":[{"name":"Franz Volhard Clinic, Virchow University Hospitals, Berlin,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. C.","family":"Luft","sequence":"additional","affiliation":[{"name":"Franz Volhard Clinic, Virchow University Hospitals, Berlin,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Haller","sequence":"additional","affiliation":[{"name":"Franz Volhard Clinic, Virchow University Hospitals, Berlin,Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1997.273.1.C161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:34:36Z","timestamp":1718652876000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1997.273.1.C161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,7,1]]}},"alternative-id":["10.1152\/ajpcell.1997.273.1.C161"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1997.273.1.c161","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7,1]]}}}