{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:28:07Z","timestamp":1761060487640,"version":"3.41.0"},"reference-count":21,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:p>Ciliary epithelial cells possess multiple purinergic receptors, and occupancy of A<jats:sub>1<\/jats:sub>and A<jats:sub>2<\/jats:sub>adenosine receptors is associated with opposing effects on intraocular pressure. Aqueous adenosine produced increases in short-circuit current across rabbit ciliary epithelium, blocked by removing Cl<jats:sup>\u2212<\/jats:sup>and enhanced by aqueous Ba<jats:sup>2+<\/jats:sup>. Adenosine\u2019s actions were further studied with nonpigmented ciliary epithelial (NPE) cells from continuous human HCE and ODM lines and freshly dissected bovine cells. With gramicidin present, adenosine (\u22653 \u03bcM) triggered isosmotic shrinkage of the human NPE cells, which was inhibited by the Cl<jats:sup>\u2212<\/jats:sup>channel blockers 5-nitro-2-(3-phenylpropylamino)benzoate (NPPB) and niflumic acid. At 10 \u03bcM, the nonmetabolizable analog 2-chloroadenosine and AMP also produced shrinkage, but not inosine, UTP, or ATP. 2-Chloroadenosine (\u22651 \u03bcM) triggered increases of whole cell currents in HCE cells, which were partially reversible, Cl<jats:sup>\u2212<\/jats:sup>dependent, and reversibly inhibited by NPPB. Adenosine (\u226510 \u03bcM) also stimulated whole cell currents in bovine NPE cells. We conclude that occupancy of adenosine receptors stimulates Cl<jats:sup>\u2212<\/jats:sup>secretion in mammalian NPE cells.<\/jats:p>","DOI":"10.1152\/ajpcell.1997.273.4.c1354","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:10:48Z","timestamp":1514135448000},"page":"C1354-C1361","source":"Crossref","is-referenced-by-count":36,"title":["Adenosine stimulates Cl<sup>\u2212<\/sup>channels of nonpigmented ciliary epithelial cells"],"prefix":"10.1152","volume":"273","author":[{"given":"David A.","family":"Carr\u00e9","sequence":"first","affiliation":[{"name":"Departments of Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire H.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Departments of Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Peterson-Yantorno","sequence":"additional","affiliation":[{"name":"Departments of Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Coca-Prados","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut 06510"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mortimer M.","family":"Civan","sequence":"additional","affiliation":[{"name":"Departments of Physiology and"},{"name":"Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania 19104-6085; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233949"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1139\/y95-044"},{"key":"B6","first-page":"2876","volume":"35","author":"Civan M. 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