{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:47:40Z","timestamp":1790218060557,"version":"4.1.0"},"reference-count":28,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:p> Nitric oxide (NO) has been implicated in endogenous control of myocardial contractility. However, NO release has not yet been demonstrated in cardiac myocytes. Accordingly, endogenous NO production was measured with a porphyrinic microsensor positioned on the surface of individual neonatal or adult rat ventricular myocytes ( n &gt; 6 neonatal and adult cells per experiment). In beating neonatal myocytes, there was no detectable spontaneous NO release with each contraction. However, norepinephrine (NE; 0.25\u20131 \u03bcM) elicited transient NO release from beating neonatal (149 \u00b1 11 to 767 \u00b1 83 nM NO) and noncontracting adult (157 \u00b1 13 to 791 \u00b1 89 nM NO) cells. NO was released by adrenergic agonists with the following rank order of potency: isoproterenol (\u03b2<jats:sub>1<\/jats:sub>\u03b2<jats:sub>2<\/jats:sub>) &gt; NE (\u03b1\/\u03b2<jats:sub>1<\/jats:sub>) &gt; dobutamine (\u03b2<jats:sub>1<\/jats:sub>) \u2248 epinephrine (\u03b1\/\u03b2<jats:sub>1<\/jats:sub>\u03b2<jats:sub>2<\/jats:sub>) &gt; tertbutylene (\u03b2<jats:sub>2<\/jats:sub>); NO was not released by phenylephrine (\u03b1). NE-evoked NO release was reversibly blocked by N <jats:sup>G<\/jats:sup>-monomethyl-l-arginine, trifluoperazine, guanosine 5\u2032- O-(2-thiodiphosphate), and nifedipine but was enhanced by 3-isobutyl-1-methylxanthine (0.5 mM = 14.5 \u00b1 1.6%) and BAY K 8644 (10 \u03bcM = 11.9 \u00b1 1%). NO was also released by A-23187 (10 \u03bcM = 884 \u00b1 88 nM NO), guanosine 5\u2032- O-(3-thiotriphosphate) (1 \u03bcM = 334 \u00b1 56 nM NO), and dibutyryl adenosine 3\u2032,5\u2032-cyclic monophosphate (10\u2013100 \u03bcM = 35 \u00b1 9 to 284 \u00b1 49 nM NO) but not by ATP, bradykinin, carbachol, 8-bromoguanosine 3\u2032,5\u2032-cyclic monophosphate, or shear stress. This first functional demonstration of a constitutive NO synthase in cardiac myocytes suggests its regulation by a \u03b2-adrenergic signaling pathway and may provide a novel mechanism for the coronary artery vasodilatation and enhanced diastolic relaxation observed with adrenergic stimulation. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.273.4.c1371","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:10:48Z","timestamp":1514135448000},"page":"C1371-C1377","source":"Crossref","is-referenced-by-count":95,"title":["\u03b2-Adrenergic regulation of constitutive  nitric oxide synthase in cardiac myocytes"],"prefix":"10.1152","volume":"273","author":[{"given":"Anthony J.","family":"Kanai","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Mesaros","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Oakland University, Rochester, Michigan 48309"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell S.","family":"Finkel","sequence":"additional","affiliation":[{"name":"Division of Cardiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmine V.","family":"Oddis","sequence":"additional","affiliation":[{"name":"Division of Cardiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori A.","family":"Birder","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of North Carolina-Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadeusz","family":"Malinski","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of North Carolina-Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14582"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47024-X"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118622"},{"issue":"34","key":"B4","first-page":"H176","volume":"265","author":"Brady A. 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