{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:37:52Z","timestamp":1773290272300,"version":"3.50.1"},"reference-count":44,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,11,1]]},"abstract":"<jats:p>The objective of this study was to evaluate the effects of nitric oxide (NO) on H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-mediated endothelial permeability. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(0.1 mM) increased permeability at 90 min to 298% of baseline. Spermine NONOate (SNO), an NO donor, at 0.1 or 1 mM did not alter permeability. However, 0.1 mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>+ 1 mM SNO increased permeability to 764%, twice that of 0.1 mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>alone. These treatments were not directly toxic to endothelial cells. This NO effect was concentration dependent, inasmuch as 0.1 mM SNO did not significantly change H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-mediated permeability. The NO-enhanced, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-dependent permeability required the simultaneous presence of NO and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, inasmuch as preincubation with SNO for 30 min followed by 0.1 mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>did not alter permeability. Staining of endothelial junctions showed widening of the intercellular space only in junctions of cells exposed to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(0.1 mM) + SNO (1 mM). Furthermore, NO did not affect H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>metabolism by endothelial cells but significantly depleted intracellular glutathione. This reduction of cell glutathione produced by NO exposure recovered 15\u201330 min after removal of the NO donor. NO-enhanced permeability was completely blocked by methionine (1 mM), a scavenger of reactive oxygen species, and by the iron chelator desferrioxamine (0.1 mM). These results suggest that NO may exacerbate the effects of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-dependent increase in endothelial monolayer permeability via the iron-catalyzed formation of reactive oxygen metabolites.<\/jats:p>","DOI":"10.1152\/ajpcell.1997.273.5.c1581","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:14:53Z","timestamp":1514135693000},"page":"C1581-C1587","source":"Crossref","is-referenced-by-count":30,"title":["Nitric oxide enhances hydrogen peroxide-mediated endothelial permeability in vitro"],"prefix":"10.1152","volume":"273","author":[{"given":"Naotsuka","family":"Okayama","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher G.","family":"Kevil","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loisann","family":"Correia","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Jourd\u2019Heuil","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Itoh","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya City University Medical School, Nagoya 467, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew B.","family":"Grisham","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Steven","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130-3932; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041560321"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(88)90085-4"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.4.1620"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13487.x"},{"key":"B5","first-page":"882","volume":"61","author":"Beutler E.","year":"1963","journal-title":"J. Lab. Clin. Med."},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1042\/bj2970209"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.76.2.199"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.5.897"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.11.6144"},{"key":"B10","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/S0022-3565(25)23789-2","volume":"271","author":"Grisham M. B.","year":"1994","journal-title":"J. Pharm. Exp. Ther."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1989.67.5.2032"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90436-Z"},{"issue":"13","key":"B13","first-page":"L403","volume":"269","author":"Hirata A.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90457-3"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1126\/science.2834821"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107470"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.75.3.528"},{"issue":"40","key":"B18","first-page":"H614","volume":"271","author":"Johnston B.","year":"1996","journal-title":"Am. J. Physiol."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(91)90452-O"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1970.tb39294.x"},{"issue":"32","key":"B21","first-page":"G34","volume":"269","author":"Kubes P.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.76.1.30"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13442.x"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1021\/jm00115a013"},{"key":"B25","first-page":"564","volume":"53","author":"Maragos C. M.","year":"1993","journal-title":"Cancer Res."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1164\/arrd.1984.130.2.209"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(96)00271-3"},{"issue":"9","key":"B28","first-page":"L613","volume":"265","author":"Meharg J. V.","year":"1993","journal-title":"Am. J. Physiol."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.1.40"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1042\/bj3100149"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.6.1133"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(94)00116-H"},{"issue":"12","key":"B33","first-page":"L699","volume":"268","author":"Pryor W. A.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.3.605.605"},{"key":"B35","first-page":"24","volume":"67","author":"Siflinger-Birnboim A.","year":"1992","journal-title":"Lab. Invest."},{"issue":"14","key":"B36","first-page":"L973","volume":"270","author":"Siflinger-Birnboim A.","year":"1996","journal-title":"Am. J. Physiol."},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227877"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1994.62062269.x"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0304"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(95)03557-5"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1021\/tx00031a003"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.9813"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb21812.x"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1021\/tx00040a007"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1997.273.5.C1581","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:15:02Z","timestamp":1751174102000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1997.273.5.C1581"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11,1]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1997,11,1]]}},"alternative-id":["10.1152\/ajpcell.1997.273.5.C1581"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1997.273.5.c1581","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11,1]]}}}