{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:07:03Z","timestamp":1718669223136},"reference-count":34,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1997,11,1]]},"abstract":"<jats:p> Aqueous humor secretion is in part linked to [Formula: see text]transport by nonpigmented ciliary epithelium (NPE) cells. During this process, the cells must maintain stable cytoplasmic pH (pH<jats:sub>i<\/jats:sub>). Because a recent report suggests that NPE cells have a plasma membrane-localized vacuolar H<jats:sup>+<\/jats:sup>-ATPase, the present study was conducted to examine whether vacuolar H<jats:sup>+<\/jats:sup>-ATPase contributes to pH<jats:sub>i<\/jats:sub> regulation in a rabbit NPE cell line. Western blot confirmed vacuolar H<jats:sup>+<\/jats:sup>-ATPase expression as judged by H<jats:sup>+<\/jats:sup>-ATPase 31-kDa immunoreactive polypeptide in both cultured NPE and native ciliary epithelium. pH<jats:sub>i<\/jats:sub> was measured using 2\u2032,7\u2032-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Exposing cultured NPE to K<jats:sup>+<\/jats:sup>-rich solution caused a pH<jats:sub>i<\/jats:sub> increase we interpret as depolarization-induced alkalinization. Alkalinization was also caused by ouabain or BaCl<jats:sub>2<\/jats:sub>. Bafilomycin A<jats:sub>1<\/jats:sub> (0.1 \u03bcM; an inhibitor of vacuolar H<jats:sup>+<\/jats:sup>-ATPase) inhibited the pH<jats:sub>i<\/jats:sub> increase caused by high K<jats:sup>+<\/jats:sup>. The pH<jats:sub>i<\/jats:sub> increase was also inhibited by angiotensin II and the metabolic uncoupler carbonyl cyanide m-chlorophenylhydazone but not by ZnCl<jats:sub>2<\/jats:sub>, 4-acetamido-4\u2032-isothiocyanostilbene-2,2\u2032-disulfonic acid (SITS), 4,4\u2032-diisothiocyanostilbene-2,2\u2032-disulfonic acid (DIDS), omeprazole, low-Cl<jats:sup>\u2212<\/jats:sup>medium, [Formula: see text]-free medium, or Na<jats:sup>+<\/jats:sup>-free medium. Bafilomycin A<jats:sub>1<\/jats:sub> slowed the pH<jats:sub>i<\/jats:sub> increase after an NH<jats:sub>4<\/jats:sub>Cl (10 mM) prepulse. However, no detectable pH<jats:sub>i<\/jats:sub> change was observed in cells exposed to bafilomycin A<jats:sub>1<\/jats:sub> under control conditions. These studies suggest that vacuolar H<jats:sup>+<\/jats:sup>-ATPase is activated by cytoplasmic acidification and by reduction of the proton electrochemical gradient across the plasma membrane. We speculate that the mechanism might contribute to maintenance of acid-base balance in NPE. <\/jats:p>","DOI":"10.1152\/ajpcell.1997.273.5.c1700","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:14:53Z","timestamp":1514135693000},"page":"C1700-C1706","source":"Crossref","is-referenced-by-count":5,"title":["Influence of bafilomycin A<sub>1<\/sub>on pH<sub>i<\/sub> responses  in cultured rabbit nonpigmented ciliary epithelium"],"prefix":"10.1152","volume":"273","author":[{"given":"Qiang","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas A.","family":"Delamere","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology and"},{"name":"Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Louisville, Kentucky 40292"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.02.110186.001143"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.1.79"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018339"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.1.C171"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115268"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1007\/BF00354085"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.260.3.C618"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.7972"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp017965"},{"key":"B10","first-page":"2511","volume":"32","author":"Delamere N. 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