{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:40:01Z","timestamp":1791092401082,"version":"4.1.0"},"reference-count":28,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1998,1,1]]},"abstract":"<jats:p>To examine the effect of aldosterone on sarcolemmal Na<jats:sup>+<\/jats:sup>transport, we measured ouabain-sensitive electrogenic Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>pump current ( I<jats:sub>p<\/jats:sub>) in voltage-clamped ventricular myocytes and intracellular Na<jats:sup>+<\/jats:sup>activity ([Formula: see text]) in right ventricular papillary muscles. Aldosterone (10 nM) induced an increase in both I<jats:sub>p<\/jats:sub>and the rate of rise of [Formula: see text] during Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>pump blockade with the fast-acting cardiac steroid dihydroouabain. The aldosterone-induced increase in I<jats:sub>p<\/jats:sub>and rate of rise of [Formula: see text] was eliminated by bumetanide, suggesting that aldosterone activates Na<jats:sup>+<\/jats:sup>influx through the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter. To obtain independent support for this, the Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, and Cl<jats:sup>\u2212<\/jats:sup>concentrations in the superfusate and solution of pipettes used to voltage clamp myocytes were set at levels designed to abolish the inward electrochemical driving force for the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter. This eliminated the aldosterone-induced increase in I<jats:sub>p<\/jats:sub>. We conclude that in vitro exposure of cardiac myocytes to aldosterone activates the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter to enhance Na<jats:sup>+<\/jats:sup>influx and stimulate the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>pump.<\/jats:p>","DOI":"10.1152\/ajpcell.1998.274.1.c175","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:49:48Z","timestamp":1514134188000},"page":"C175-C181","source":"Crossref","is-referenced-by-count":64,"title":["Na<sup>+<\/sup>influx and Na<sup>+<\/sup>-K<sup>+<\/sup>pump activation during short-term exposure of cardiac myocytes to aldosterone"],"prefix":"10.1152","volume":"274","author":[{"given":"Anastasia S.","family":"Mihailidou","sequence":"first","affiliation":[{"name":"Department of Cardiology,Royal North Shore Hospital, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerrie A.","family":"Buhagiar","sequence":"additional","affiliation":[{"name":"Department of Cardiology,Royal North Shore Hospital, and"},{"name":"University of Sydney, Sydney, New South Wales 2065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helge H.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Cardiology,Royal North Shore Hospital, and"},{"name":"University of Sydney, Sydney, New South Wales 2065, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp017931"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.4.C878"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/science.2935937"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/26.5.433"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.260.4.C681"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.4.741"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.100.1.89"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90566-8"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1990.259.1.F40"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/315063a0"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.94.3.511"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.4.C1186"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.2.C366"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99065-4"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/BF00220767"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.92.2.175"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.71.3.503"},{"key":"B19","first-page":"71","volume":"2","author":"MacLeod K. 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