{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:19:18Z","timestamp":1773458358378,"version":"3.50.1"},"reference-count":43,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1998,8,1]]},"abstract":"<jats:p> Monolayers of the human colonic epithelial cell line T84 exhibit electrogenic Cl<jats:sup>\u2212<\/jats:sup> secretion in response to the Ca<jats:sup>2+<\/jats:sup> agonist thapsigargin and to the cAMP agonist forskolin. To evaluate directly the regulation of apical Cl<jats:sup>\u2212<\/jats:sup>conductance by these two agonists, we have utilized amphotericin B to permeabilize selectively the basolateral membranes of T84 cell monolayers. We find that apical anion conductance is stimulated by both forskolin and thapsigargin but that these conductances are differentially sensitive to the anion channel blocker DIDS. DIDS inhibits thapsigargin-stimulated responses completely but forskolin responses only partially. Furthermore, the apical membrane anion conductances elicited by these two agonists differ in anion selectivity (for thapsigargin, I<jats:sup>\u2212<\/jats:sup> &gt; Cl<jats:sup>\u2212<\/jats:sup>; for forskolin, Cl<jats:sup>\u2212<\/jats:sup> &gt; I<jats:sup>\u2212<\/jats:sup>). However, the DIDS-sensitive component of the forskolin-induced conductance response exhibits anion selectivity similar to that induced by thapsigargin (I<jats:sup>\u2212<\/jats:sup> &gt; Cl<jats:sup>\u2212<\/jats:sup>). Thus forskolin-induced apical anion conductance comprises at least two components, one of which has features in common with that elicited by thapsigargin. <\/jats:p>","DOI":"10.1152\/ajpcell.1998.275.2.c484","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:49:58Z","timestamp":1514137798000},"page":"C484-C495","source":"Crossref","is-referenced-by-count":62,"title":["Distinct Ca<sup>2+<\/sup>- and cAMP-dependent anion conductances  in the apical membrane of polarized T84 cells"],"prefix":"10.1152","volume":"275","author":[{"given":"Didier","family":"Merlin","sequence":"first","affiliation":[{"name":"Division of Gastrointestinal Pathology, Department of Pathology, Brigham and Women\u2019s Hospital,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianwei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregg R.","family":"Strohmeier","sequence":"additional","affiliation":[{"name":"Division of Gastrointestinal Pathology, Department of Pathology, Brigham and Women\u2019s Hospital,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asma","family":"Nusrat","sequence":"additional","affiliation":[{"name":"Division of Gastrointestinal Pathology, Department of Pathology, Brigham and Women\u2019s Hospital,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seth L.","family":"Alper","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne I.","family":"Lencer","sequence":"additional","affiliation":[{"name":"Combined Program in Pediatric Gastroenterology and Nutrition, Department of Medicine, Children\u2019s Hospital Medical Center,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Madara","sequence":"additional","affiliation":[{"name":"Division of Gastrointestinal Pathology, Department of Pathology, Brigham and Women\u2019s Hospital,"},{"name":"Harvard Medical School and Harvard Digestive Diseases Center, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.14.6003"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233281"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.4.C859"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.4.C1069"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.4.C958"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.3.C555"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869947"},{"issue":"27","key":"B8","first-page":"G325","volume":"264","author":"Brayden D. 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