{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:56:03Z","timestamp":1773420963735,"version":"3.50.1"},"reference-count":31,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1998,10,1]]},"abstract":"<jats:p> Cells of a newly described, immortalized, epithelial, human transverse colonic cell line, NCM460, reach \u223c90% confluence on plastic and develop transepithelial resistances of 120\u2013250 \u03a9 \u22c5 cm<jats:sup>2<\/jats:sup> on porous substrates. Its utility as a model for the transverse human colon was validated by comparing second messenger-mediated Cl<jats:sup>\u2212<\/jats:sup> transport, using the fluorescent probe 6-methoxy-quinolyl acetoethyl ester, in NCM460 cells and colonocytes isolated from human transverse crypts. Basal Cl<jats:sup>\u2212<\/jats:sup> influx was increased ( P &lt; 0.01) by PGE<jats:sub>1<\/jats:sub> (1 \u03bcM), forskolin (1 \u03bcM), 8-bromoadenosine 3\u20325\u2032-cyclic monophosphate (100 \u03bcM), heat-stable Escherichia colienterotoxin (STa; 1 \u03bcM), 8-bromoguanosine 3\u20325\u2032-cyclic monophosphate (100 \u03bcM), histamine (1 \u03bcM), and phorbol 12,13-dibutyrate (1 \u03bcM) in both cell types. The Cl<jats:sup>\u2212<\/jats:sup> channel blocker diphenylamine 2-carboxylic acid (50 \u03bcM) and the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransport inhibitor furosemide (1 \u03bcM), but not the K<jats:sup>+<\/jats:sup> channel blocker Ba<jats:sup>2+<\/jats:sup> (3 mM), inhibited these Cl<jats:sup>\u2212<\/jats:sup> permeabilities. These cells possess transcripts for cystic fibrosis transmembrane conductance regulator, Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter, STa receptor, and intestine-specific cGMP-dependent protein kinase II. Thus cAMP-, cGMP-, and Ca<jats:sup>2+<\/jats:sup>-dependent secretagogues act on NCM460 and primary colonocytes to stimulate Cl<jats:sup>\u2212<\/jats:sup> transport. This validates the utility of NCM460 as a model for transverse colonic crypts and is the first demonstration of a colonic cell line whose origin is known. <\/jats:p>","DOI":"10.1152\/ajpcell.1998.275.4.c1048","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:42:52Z","timestamp":1514137372000},"page":"C1048-C1057","source":"Crossref","is-referenced-by-count":21,"title":["Cl<sup>\u2212<\/sup> transport in an immortalized human epithelial cell  line (NCM460) derived from the normal transverse colon"],"prefix":"10.1152","volume":"275","author":[{"given":"Jasminder","family":"Sahi","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics and"},{"name":"Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selvaraj G.","family":"Nataraja","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J.","family":"Layden","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay L.","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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