{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T19:15:56Z","timestamp":1773256556154,"version":"3.50.1"},"reference-count":41,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1998,11,1]]},"abstract":"<jats:p> We examined the effects of human cytomegalovirus (HCMV) infection on the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NKCC) in a human fibroblast cell line. Using the Cl<jats:sup>\u2212<\/jats:sup>-sensitive dye MQAE, we showed that the mock-infected MRC-5 cells express a functional NKCC. 1) Intracellular Cl<jats:sup>\u2212<\/jats:sup> concentration ([Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>i<\/jats:sub>) was significantly reduced from 53.4 \u00b1 3.4 mM to 35.1 \u00b1 3.6 mM following bumetanide treatment. 2) Net Cl<jats:sup>\u2212<\/jats:sup> efflux caused by replacement of external Cl<jats:sup>\u2212<\/jats:sup>with gluconate was bumetanide sensitive. 3) In Cl<jats:sup>\u2212<\/jats:sup>-depleted mock-infected cells, the Cl<jats:sup>\u2212<\/jats:sup> reuptake rate (in [Formula: see text]-free media) was reduced in the absence of external Na<jats:sup>+<\/jats:sup> and by treatment with bumetanide. After HCMV infection, we found that although [Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>i<\/jats:sub>increased progressively [24 h postexposure (PE), 65.2 \u00b1 4.5 mM; 72 h PE, 80.4 \u00b1 5.0 mM], the bumetanide and Na<jats:sup>+<\/jats:sup> sensitivities of [Cl<jats:sup>\u2212<\/jats:sup>]<jats:sub>i<\/jats:sub>and net Cl<jats:sup>\u2212<\/jats:sup> uptake and loss were reduced by 24 h PE and abolished by 72 h PE. Western blots using the NKCC-specific monoclonal antibody T4 showed an approximately ninefold decrease in the amount of NKCC protein after 72 h of infection. Thus HCMV infection resulted in the abolition of NKCC function coincident with the severe reduction in the amount of NKCC protein expressed. <\/jats:p>","DOI":"10.1152\/ajpcell.1998.275.5.c1330","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:31:27Z","timestamp":1514136687000},"page":"C1330-C1341","source":"Crossref","is-referenced-by-count":21,"title":["Na<sup>+<\/sup>-K<sup>+<\/sup>-Cl<sup>\u2212<\/sup>cotransport in human fibroblasts is  inhibited by cytomegalovirus infection"],"prefix":"10.1152","volume":"275","author":[{"given":"Lilia M.","family":"Maglova","sequence":"first","affiliation":[{"name":"Department of Physiology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19129; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E.","family":"Crowe","sequence":"additional","affiliation":[{"name":"Department of Physiology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19129; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Physiology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19129; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An\u00edbal A.","family":"Altamirano","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Cardiol\u00f3gicas, Facultad de Medicina, Universidad de Buenos Aires, 1122 Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Physiology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19129; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1675-4_6"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1107"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(94)00230-C"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)36720-0"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-215-44114"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.4.C749"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0516"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/264807a0"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(89)82755-9"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.2.C402"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1739"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-196-43167"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.4.C869"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.16.9666"},{"issue":"36","key":"B16","first-page":"H2114","volume":"267","author":"Koncz C.","year":"1994","journal-title":"Am. 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