{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:09:08Z","timestamp":1790867348532,"version":"4.1.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1999,1,1]]},"abstract":"<jats:p> We investigated the relationship between pI<jats:sub>Cln<\/jats:sub>, the volume-activated Cl<jats:sup>\u2212<\/jats:sup>current, and volume regulation in native bovine nonpigmented ciliary epithelial (NPCE) cells. Immunofluorescence studies demonstrated the presence of pI<jats:sub>Cln<\/jats:sub> protein in the NPCE cells. Exposure to hypotonic solution activated a Cl<jats:sup>\u2212<\/jats:sup> current and induced regulatory volume decrease (RVD) in freshly isolated bovine NPCE cells. Three antisense oligonucleotides complementary to human pI<jats:sub>Cln<\/jats:sub> mRNA were used in the experiments. The antisense oligonucleotides were taken up by the cells in a dose-dependent manner. The antisense oligonucleotides, designed to be complementary to the initiation codon region of the human pI<jats:sub>Cln<\/jats:sub> mRNA, \u201cknocked down\u201d the pI<jats:sub>Cln<\/jats:sub> protein immunofluorescence, delayed the activation of volume-activated Cl<jats:sup>\u2212<\/jats:sup> current, diminished the value of the current, and reduced the ability of the cells to volume regulate. We conclude that pI<jats:sub>Cln<\/jats:sub>is involved in the activation pathway of the volume-activated Cl<jats:sup>\u2212<\/jats:sup> current and RVD following hypotonic swelling. <\/jats:p>","DOI":"10.1152\/ajpcell.1999.276.1.c182","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T17:34:57Z","timestamp":1514136897000},"page":"C182-C192","source":"Crossref","is-referenced-by-count":62,"title":["Association of intrinsic pI<sub>Cln<\/sub> with volume-activated Cl<sup>\u2212<\/sup>  current and volume regulation in a native epithelial cell"],"prefix":"10.1152","volume":"276","author":[{"given":"Lixin","family":"Chen","sequence":"first","affiliation":[{"name":"School of Biosciences, Cardiff University, Cardiff CF1 3US, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biosciences, Cardiff University, Cardiff CF1 3US, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim J. 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