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Results indicate that the rat lacrimal gland does not contain detectable soluble and mature EGF but that the EGF immunoreactivity is associated with the membrane-enriched fraction. Analysis of the detergent-solubilized membrane proteins by gel filtration shows that membrane-associated EGF immunoreactivity was present as a high-molecular-mass protein. Moreover, as shown by Western blot analysis, a specific anti-rat EGF precursor antibody (ppEGF<jats:sub>1<\/jats:sub>) can immunoprecipitate a 152-kDa EGF-containing protein. Taken together, these results demonstrate for the first time both EGF precursor gene transcription and EGF precursor protein expression in a lacrimal tissue, i.e., the rat exorbital lacrimal gland. The demonstration that EGF appears to be stored only as its full-length membrane precursor may provide important information to study the regulation of its secretory process. <\/jats:p>","DOI":"10.1152\/ajpcell.1999.276.3.c734","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T22:22:28Z","timestamp":1514154148000},"page":"C734-C746","source":"Crossref","is-referenced-by-count":13,"title":["EGF precursor mRNA and membrane-associated EGF  precursor protein in rat exorbital lacrimal gland"],"prefix":"10.1152","volume":"276","author":[{"given":"Herv\u00e9","family":"Mar\u00e9chal","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie des Transports Cellulaires, Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 5619, Universit\u00e9 Paris-Sud, 91405 Orsay Cedex; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9lene","family":"Jammes","sequence":"additional","affiliation":[{"name":"Unit\u00e9d\u2019Endocrinologie Mol\u00e9culaire, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Rossignol","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Transports Cellulaires, Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 5619, Universit\u00e9 Paris-Sud, 91405 Orsay Cedex; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Mauduit","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Transports Cellulaires, Centre National de la Recherche Scientifique, Unit\u00e9 Mixte de Recherche 5619, Universit\u00e9 Paris-Sud, 91405 Orsay Cedex; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.21.8427"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46260-0"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1210\/mend-3-7-1077"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.27.17258"},{"key":"B5","first-page":"263","volume":"1","author":"Burstein N. 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